| Autor: |
J. Ivan Legg, Brian Warner, Gerhard Boehme, Mickey S. Urdea, Karl H. Pool |
| Rok vydání: |
1980 |
| Předmět: |
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| Zdroj: |
Analytical Biochemistry. 106:175-185 |
| ISSN: |
0003-2697 |
| DOI: |
10.1016/0003-2697(80)90134-7 |
| Popis: |
A sensitive, inexpensively constructed pH stat-autotitrator is described. In the pH stat mode of operation a digitally controlled buret adds titrant to a reaction mixture at a rate proportional to the differences between the solution pH and the stat pH. The digital buret employs inexpensive Hamilton glass syringes (250 μl → 2.5 ml) to deliver titrant at rates ranging from ∼ 100 nl/min to ∼ 1.5 ml/min. Both the volume of titrant and the pH are displayed digitally and are available as an analog signal for use by an external chart recorder. An equation relating reaction parameters (reaction volume, titrant normality, buffer capacity, and kinetic constants) to tracking accuracy is derived. The predictions of the derived relationship are of general importance to all p X -based kinetic analyses. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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