Chronic and Intermittent Hyperglycemia Modulates Expression of Key Molecules of PI3K/AKT Pathway in Differentiating Human Visceral Adipocytes
| Autor: | Justyna Strycharz, Adam Wróblewski, Józef Drzewoski, Janusz Szemraj, Piotr Czarny, Ewa Świderska, Agnieszka Śliwińska |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
PTEN IRS1 Gene Expression Adipose tissue PI3K Phosphatidylinositol 3-Kinases 0302 clinical medicine Insulin Glucose homeostasis Biology (General) Spectroscopy Glucose Transporter Type 4 biology diabetes Chemistry miR Cell Differentiation General Medicine Computer Science Applications Adipogenesis Female Signal Transduction medicine.medical_specialty QH301-705.5 adipocytes Primary Cell Culture 030209 endocrinology & metabolism Intra-Abdominal Fat Article Catalysis adipogenesis Inorganic Chemistry 03 medical and health sciences Internal medicine medicine Humans Physical and Theoretical Chemistry QD1-999 Molecular Biology Protein kinase B PI3K/AKT/mTOR pathway Cell Proliferation AKT2 Organic Chemistry PTEN Phosphohydrolase MicroRNAs Insulin receptor Glucose 030104 developmental biology Endocrinology Insulin Receptor Substrate Proteins biology.protein hyperglycemia Insulin Resistance Proto-Oncogene Proteins c-akt GLUT4 |
| Zdroj: | International Journal of Molecular Sciences Volume 22 Issue 14 International Journal of Molecular Sciences, Vol 22, Iss 7712, p 7712 (2021) |
| ISSN: | 1422-0067 |
| DOI: | 10.3390/ijms22147712 |
| Popis: | Background: Due to its prominence in the regulation of metabolism and inflammation, adipose tissue is a major target to investigate alterations in insulin action. This hormone activates PI3K/AKT pathway which is essential for glucose homeostasis, cell differentiation, and proliferation in insulin-sensitive tissues, like adipose tissue. The aim of this work was to evaluate the impact of chronic and intermittent high glucose on the expression of biomolecules of insulin signaling pathway during the differentiation and maturation of human visceral preadipocytes. Methods: Human visceral preadipocytes (HPA-V) cells were treated with high glucose (30 mM)during the proliferation and/or differentiation and/or maturation stage. The level of mRNA (by Real-Time PCR) and protein (by Elisa tests) expression of IRS1, PI3K, PTEN, AKT2, and GLUT4 was examined after each culture stage. Furthermore, we investigated whether miR-29a-3p, miR-143-3p, miR-152-3p, miR-186-5p, miR-370-3p, and miR-374b-5p may affect the expression of biomolecules of the insulin signaling pathway. Results: Both chronic and intermittent hyperglycemia affects insulin signaling in visceral pre/adipocytes by upregulation of analyzed PI3K/AKT pathway molecules. Both mRNA and protein expression level is more dependent on stage-specific events than the length of the period of high glucose exposure. What is more, miRs expression changes seem to be involved in PI3K/AKT expression regulation in response to hyperglycemic stimulation. |
| Databáze: | OpenAIRE |
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