Helianthus tuberosus agglutinin directly induces neutrophil migration, which can be modulated/inhibited by resident mast cells
| Autor: | Nylane M.N. Alencar, Veruska B.M. Alencar, Edson Holanda Teixeira, Ana Maria Sampaio Assreuy, Benildo Sousa Cavada, Vicente P. T. Pinto, Emmanuel P. Souza, Gerly Anne de Castro Brito, Henri Debray, Ronaldo A. Ribeiro |
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| Rok vydání: | 2005 |
| Předmět: |
Male
Dose-Response Relationship Drug Helianthus tuberosus agglutinin Neutrophils Chemistry Peritonitis Inflammation Cell Biology medicine.disease Biochemistry Molecular biology In vitro Rats Chemotaxis Leukocyte In vivo Macrophages Peritoneal medicine Animals Female Mast Cells NEUTROPHIL MIGRATION Plant Lectins Rats Wistar medicine.symptom Molecular Biology |
| Zdroj: | Biochemistry and Cell Biology. 83:659-666 |
| ISSN: | 1208-6002 0829-8211 |
| DOI: | 10.1139/o05-118 |
| Popis: | We investigated the effect of Helianthus tuberosus agglutinin (HTA) on neutrophil migration in vivo and in vitro. The role of resident cells in this effect was analyzed. Peritonitis was induced by injecting stimuli into rat (150–200 g) peritoneal cavities, and in vitro neutrophil chemotaxis was performed using a Boyden microchamber. HTA (80, 200, or 500 µg/mL per cavity) induced significant in vivo neutrophil migration (p < 0.05); in vitro assays showed that this lectin also induced neutrophil chemotaxis, an effect inhibited by the incubation of lectin associated with α-D(+)-mannose, its specific binding sugar. Depletion of the resident-cell population by peritoneal lavage did not alter HTA-induced neutrophil migration (200 µg/mL per cavity). The opposite strategy, increasing peritoneal macrophages by intraperitoneally injecting rats with thioglycollate, did not enhance the neutrophil migration produced by HTA (200 µg/mL per cavity). In addition, injection of supernatant from HTA-stimulated macrophage culture (300 µg/mL) into rat peritoneal cavities did not induce neutrophil migration. However, reduction of the peritoneal mast-cell population potentiated the neutrophil migration (p < 0.05) induced by HTA (200 µg/mL per cavity). Lectin from H. tuberosus has a direct neutrophil chemotatic effect that is modulated by mast cells.Key words: lectins, inflammation, Helianthus tuberosus, neutrophil migration. |
| Databáze: | OpenAIRE |
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