Evaluating the biochemical changes of LPS-stimulated endothelial cells by synchrotron FTIR microspectroscopy at a single-cell level

Autor:	Yue Wang, Lina Huang, Yadi Wang, Junhong Lü, Xiangyong Liu, Jun Hu
Rok vydání:	2019
Předmět:	Lipopolysaccharides Lipopolysaccharide 02 engineering and technology 01 natural sciences Biochemistry Analytical Chemistry Glycocalyx chemistry.chemical_compound Single-cell analysis Spectroscopy Fourier Transform Infrared Human Umbilical Vein Endothelial Cells Humans Fourier transform infrared spectroscopy chemistry.chemical_classification Chemistry 010401 analytical chemistry Fatty acid 021001 nanoscience & nanotechnology 0104 chemical sciences Endothelial stem cell Membrane Biophysics Nucleic acid lipids (amino acids peptides and proteins) Endothelium Vascular Single-Cell Analysis 0210 nano-technology Synchrotrons
Zdroj:	Analytical and Bioanalytical Chemistry. 411:7157-7164
ISSN:	1618-2650 1618-2642
Popis:	Endothelial damage is a major manifestation in many forms of heart and lung injuries induced by lipopolysaccharide (LPS), but the biochemical responses and activation mechanisms of endothelial cells have not been fully explicit. In this study, the biochemical changes to endothelial cells exposed to LPS were investigated by synchrotron FTIR microspectroscopy at a single-cell level. We found that the whole infrared spectrum of endothelial cells shifted after LPS treatment, indicating chemical component changes within cells. Principal component analysis (PCA) and t tests on subspectra (fatty acid region, protein region, and nucleic acid-sugar region, respectively) further showed that sugar components as well as fatty acids changed dramatically while proteins had no significant variation following LPS exposure. These results suggested that the glycocalyx layer structure on endothelial cell membrane may be mainly influenced by LPS and also proved that synchrotron FTIR microspectroscopy was a useful technique to evaluate the biochemical changes of endothelial damage at the single-cell level. Graphical abstract.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::31666c702eb842a1fe03b390cc9801a2 https://doi.org/10.1007/s00216-019-02083-8 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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