Hybridization of EcoRI chloroplast DNA fragments of Euglena to pulse labeled RNA from different stages of chloroplast development
| Autor: | James R. Y. Rawson, Cindy L. Boerma |
|---|---|
| Rok vydání: | 1979 |
| Předmět: |
Chloroplasts
Light Transcription Genetic Biophysics EcoRI EcoRI Endonuclease Biochemistry Euglena Transcription (biology) Animals Molecular Biology biology food and beverages RNA Nucleic Acid Hybridization Cell Biology DNA DNA Restriction Enzymes biology.organism_classification Molecular biology Chloroplast Molecular Weight Kinetics Chloroplast DNA biology.protein |
| Zdroj: | Biochemical and biophysical research communications. 89(2) |
| ISSN: | 0006-291X |
| Popis: | Transcription of Euglena chloroplast DNA was visualized by autoradiography of hybrids formed between electrophoretically separated EcoRI endonuclease fragments and [32P]-pulse labeled RNA prepared from cells at different stages of chloroplast development. The RNA was hybridized to Southern imprints of EcoRI chloroplast DNA digests. The EcoRI chloroplast DNA fragments were categorized by their ability to hybridize the different pulse labeled RNA's. Some fragments were transcribed: 1. both in the dark and throughout chloroplast development, 2. predominantly in the light, 3. in the dark and for only a brief period of time after transfer of the cells to the light. Other fragments were probably not transcribed. |
| Databáze: | OpenAIRE |
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