Effects of Feeding a Probiotic Preparation (SIM) Containing Inulin on the Severity of Colitis and on the Composition of the Intestinal Microflora in HLA-B27 Transgenic Rats
| Autor: | Gerald W. Tannock, H. Schwietz, Jürgen Schölmerich, I. Melchner, Claudia Göttl, Michael Schultz, Heiko C. Rath, Karen Munro |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
DNA
Bacterial Microbiology (medical) Colon medicine.medical_treatment Clinical Biochemistry Immunology Inulin Polymerase Chain Reaction law.invention Microbiology Animals Genetically Modified Probiotic chemistry.chemical_compound Lactobacillus acidophilus law Experimental Clinical Investigation Metronidazole Lactobacillus medicine Animals Immunology and Allergy Colitis HLA-B27 Antigen Peroxidase Bifidobacterium Bacteria biology Probiotics Prebiotic Sequence Analysis DNA Inflammatory Bowel Diseases biology.organism_classification medicine.disease Rats Inbred F344 Rats Bifidobacterium animalis Intestines chemistry Electrophoresis Polyacrylamide Gel beta 2-Microglobulin |
| Zdroj: | Clinical and Vaccine Immunology. 11:581-587 |
| ISSN: | 1556-679X 1556-6811 |
| DOI: | 10.1128/cdli.11.3.581-587.2004 |
| Popis: | An overly aggressive immune response to the intestinal microflora in a genetically susceptible host background has been implicated in the pathogenesis of inflammatory bowel diseases. We measured the impact of a probiotic preparation (SIM) containing inulin on the severity of colitis and on intestinal microflora profiles of HLA-B27-β 2 -microglobulin transgenic (TG) rats. SIM is a mixture of lactobacilli, bifidobacteria, and inulin. Two-month-old TG rats received either SIM or water. Control TG rats received metronidazole, alone or in combination with SIM, for 8 weeks. Nontransgenic rats received SIM or water. The cecal content was removed for analysis of the intestinal microflora by PCR combined with denaturing gradient gel electrophoresis. The colon was scored for histological evidence of inflammation, colonic myeloperoxidase activity and interleukin-1β RNA levels were measured photometrically or by real-time quantitative PCR. At 4 months, the colonic inflammation of TG rats treated with SIM was histologically diminished compared to that in untreated TG rats (2.2 ± 0.2 versus 2.9 ± 0.1; P ≤ 0.03). The administration of SIM altered the microflora profiles of TG rats by increasing the diversity and stimulating specifically the growth of Bifidobacterium animalis . The probiotic bacteria added to SIM were below the detection level in cecal stool samples at the end of the study period. The administration of SIM resulted in a measurable impact on the cecal microflora profiles of TG rats with attenuation of colitis. The lack of detection of any added probiotic bacteria in the cecal content suggests that prebiotic inulin is the major effective compound. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|