Murine mast cells synthesize basement membrane components. A potential role in early fibrosis
| Autor: | Yoshihiko Yamada, Dean D. Metcalfe, Helen L. Thompson, Peter D. Burbelo, Gary C. Gabriel |
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| Rok vydání: | 1991 |
| Předmět: |
Transcription
Genetic Blotting Western Inflammation Basement Membrane Extracellular matrix Mice Laminin medicine Animals Mast Cells RNA Messenger Interleukin 3 Basement membrane Extracellular Matrix Proteins biology General Medicine Blotting Northern Mast cell Fibrosis Molecular biology Actins Epithelium Interleukin 33 medicine.anatomical_structure biology.protein Interleukin-3 Proteoglycans Collagen Heparitin Sulfate medicine.symptom Research Article |
| Zdroj: | Journal of Clinical Investigation. 87:619-623 |
| ISSN: | 0021-9738 |
| DOI: | 10.1172/jci115038 |
| Popis: | Mast cells are resident in tissues, particularly in association with endothelial and epithelial cell basement membranes, and increase at sites of inflammation, injury, and fibrosis. Although mast cells are known to both release and generate proinflammatory molecules in response to inflammatory stimuli, little is known about their normal biologic function. Here we demonstrate that IL-3-dependent mouse PT18 mast cells, mouse bone marrow-derived mast cells, and rat basophilic leukemia cells express large amounts of mRNA for collagen IV, laminin, and heparan sulfate proteoglycan. Western blot analysis confirmed that mast cells synthesize and secrete significant amounts collagen IV and laminin B1 and B2 chains. These data suggest that mast cells may contribute to normal tissue repair and/or the early overproduction of basement membrane components seen in a variety of fibrotic conditions. |
| Databáze: | OpenAIRE |
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