Potential applications of lipid peroxidation products – F4-neuroprostanes, F3-neuroprostanesn-6 DPA, F2-dihomo-isoprostanes and F2-isoprostanes ‐ in the evaluation of the allograft function in renal transplantation
| Autor: | Jean-Marie Galano, Elena Lozoya, Lidia Marín-Soler, Camille Oger, Pedro Martínez-Hernández, Teresa Casas-Pina, Sonia Medina, Thierry Durand, Luisa Jimeno, Ignacio De las Heras-Gómez, Santiago Llorente, Federico Ferreres, Angel Gil-Izquierdo, Anna Tomás |
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| Přispěvatelé: | University Hospital Virgen de la Arrixaca, CEBAS-CSIC, Research group on Quality, Safety and Bioactivity of Plant Foods, Spanish National Research Council (CSIC), Institut des Biomolécules Max Mousseron [Pôle Chimie Balard] (IBMM), Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM) |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Nephrology medicine.medical_specialty 030232 urology & nephrology Renal function Pharmacology medicine.disease_cause Biochemistry Lipid peroxidation 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine [CHIM.ANAL]Chemical Sciences/Analytical chemistry Physiology (medical) Internal medicine medicine [CHIM]Chemical Sciences Chemistry Isoprostanes 3. Good health Transplantation 030104 developmental biology Docosahexaenoic acid Neuroprostanes Oxidative stress |
| Zdroj: | Free Radical Biology and Medicine Free Radical Biology and Medicine, Elsevier, 2017, ⟨10.1016/j.freeradbiomed.2017.01.019⟩ |
| ISSN: | 0891-5849 |
| DOI: | 10.1016/j.freeradbiomed.2017.01.019 |
| Popis: | International audience; F4-neuroprostanes, F3-neuroprostanes n-6 DPA , and F2-dihomo-isoprostanes, metabolites of non-enzymatic lipid peroxidation of polyunsaturated fatty acids [docosahexaenoic acid, n-6 docosapentanoic acid, and adrenic acid respectively], have become important biomarkers for oxidative stress in several diseases like epilepsy and alzheimer. These biomarkers and the 15-F2t-isoprostane (also known as 8-iso-PGF 2α) , a F2-isoprostane isomer measured as reference oxidative marker at systemic level, were analyzed by UHPLC-QqQ-MS/MS in the urine of 60 renal recipients from cadaveric donors of the Nephrology Unit of the University Hospital Virgen de la Arrixaca, at six different times during the first six months after renal transplantation, and were compared with a control group of 60 healthy subjects from the same hospital. A total of 11 metabolites were analyzed and different patterns were observed. A tendency to decrease was observed in three metabolites (4-epi−4-F3t-NeuroP n-6 DPA , ent−7(RS)−7-F2t-dihomo-IsoP, and ent−7(S)−7-F2t-dihomo-IsoP) and in our reference oxidative marker (15-F2t-IsoP) when kidney function improved and the excretion of urine proteins decreased. These results suggest that these three biomarkers of oxidative stress could be useful to assess renal function in the postransplant phase. Unfortunately, little is known about this kind of biomarker in this cohort of patients, so further investigation would be required in the clinical field to clarify the relationship between oxidative stress and the graft function, as well as the usefulness of these biomarkers as rejection markers. |
| Databáze: | OpenAIRE |
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