A Possible Role for Caveolin as a Signaling Organizer in Olfactory Sensory Membranes
| Autor: | Heinz Breer, Jörg Fleischer, Ingrid Boekhoff, Sylvia Schreiber |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Male
G protein Immunoprecipitation Caveolin 2 Caveolin 1 Sensory system Biology Cell Fractionation Receptors Odorant Caveolins Second Messenger Systems Biochemistry Rats Sprague-Dawley Olfactory Mucosa Caveolin Cyclic AMP Animals Cilia Molecular Biology Microvilli Effector Colforsin Membrane Proteins Cell Biology Heterotrimeric GTP-Binding Proteins Peptide Fragments Rats Cell biology Guanosine 5'-O-(3-Thiotriphosphate) Odorants Second messenger system Female Vomeronasal Organ Signal transduction Transduction (physiology) Protein Binding Signal Transduction |
| Zdroj: | Journal of Biological Chemistry. 275:24115-24123 |
| ISSN: | 0021-9258 |
| Popis: | Fast kinetics and sensitivity of olfactory signaling raise the question of whether the participating proteins may be associated in supramolecular transduction complexes. We found evidence that caveolin proteins could play an important role in organizing signaling elements in olfactory sensory neurons. Western blot analysis indicated that caveolins are highly enriched in olfactory sensory membranes, where they co-localize in detergent-insoluble complexes with key components of the signaling pathways. Furthermore, the results of immunoprecipitation experiments suggest that G proteins and effector enzyme form preassembled subcellular complexes with caveolins. Since anti-caveolin antibodies and synthetic peptides derived from the scaffolding domains of caveolin-1 and caveolin-2 effectively attenuated second messenger responses in sensory cilia preparations in a characteristic manner, the data led to the suggestion that caveolins could mediate the assembly of signaling complexes within specialized membrane microdomains of olfactory sensory neurons. |
| Databáze: | OpenAIRE |
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