Effects of vitamin D-induced supernatant of placental explants from preeclamptic women on oxidative stress and nitric oxide bioavailability in human umbilical vein endothelial cells
| Autor: | Priscila Rezeck Nunes, Virginia Juliani Gomes, Maria Terezinha Serrão Peraçoli, Mattias Carlström, Valeria C. Sandrim, José Carlos Peraçoli |
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| Přispěvatelé: | Universidade Estadual Paulista (Unesp), Karolinska Inst |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Medicine (General) Physiology QH301-705.5 Placenta Immunology Biophysics Biological Availability Ocean Engineering medicine.disease_cause Nitric Oxide Biochemistry Umbilical vein Nitric oxide Preeclampsia Andrology Lipid peroxidation 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine R5-920 Pre-Eclampsia Pregnancy medicine Human Umbilical Vein Endothelial Cells Humans General Pharmacology Toxicology and Pharmaceutics Vitamin D Biology (General) Cells Cultured General Neuroscience Cell Biology General Medicine Hydrogen Peroxide medicine.disease Malondialdehyde Bioavailability 030104 developmental biology chemistry Oxidative stress Placental explants 030220 oncology & carcinogenesis Female Explant culture Research Article |
| Zdroj: | Brazilian Journal of Medical and Biological Research, Vol 54, Iss 8 (2021) Brazilian Journal of Medical and Biological Research v.54 n.8 2021 Brazilian Journal of Medical and Biological Research Associação Brasileira de Divulgação Científica (ABDC) instacron:ABDC Brazilian Journal of Medical and Biological Research, Volume: 54, Issue: 8, Article number: e11073, Published: 24 MAY 2021 Web of Science Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
| ISSN: | 0100-879X |
| Popis: | Made available in DSpace on 2021-06-25T15:06:18Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-01-01. Added 1 bitstream(s) on 2021-07-15T14:36:46Z : No. of bitstreams: 1 S0100-879X2021000800604.pdf: 632798 bytes, checksum: 7a3373bbfe0a871d577a001fd4fb9313 (MD5) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Swedish Research Council Swedish Heart and Lung Foundation NovoNordisk The study evaluated the effect of the supernatant of placental explants from preeclamptic (PE) and normotensive (NT) pregnant women after tissue treatment with or without vitamin D (VD) on oxidative stress and nitric oxide (NO) bioavailability in human umbilical vein endothelial cells (HUVEC). Placental explants were prepared from eight NT and eight PE women, and supernatants were obtained after incubation with or without hydrogen peroxide (H2O2) and/or VD. HUVEC were cultured for 24 h with supernatants, and the following parameters were analyzed in HUVEC cultures: NO, nitrate (NO3-), and nitrite (NO2-) levels, lipid peroxidation, and intracellular reactive oxygen species (ROS). Results showed that the production of NO3-, NO2-, malondialdehyde (MDA), and ROS were significantly higher in HUVEC treated with explant supernatant from PE compared to NT pregnant women, while the supernatant of PE explants treated with VD led to a decrease in these parameters. A significantly high production of NO was detected in HUVEC cultured with control supernatant of NT group, and in cultures treated with supernatant of PE explants treated with VD. Taken together, these results demonstrated that cultures of placental explants from PE women with VD treatment generated a supernatant that decreased oxidative stress and increased the bioavailability of NO in endothelial cells. Univ Estadual Paulista, Fac Med Botucatu, Dept Ginecol & Obstet, Botucatu, SP, Brazil Karolinska Inst, Dept Physiol & Pharmacol, Stockholm, Sweden Univ Estadual Paulista, Inst Biociencias, Dept Biofis & Farmacol, Botucatu, SP, Brazil Univ Estadual Paulista, Fac Med Botucatu, Dept Ginecol & Obstet, Botucatu, SP, Brazil Univ Estadual Paulista, Inst Biociencias, Dept Biofis & Farmacol, Botucatu, SP, Brazil FAPESP: 2016/23452-2 FAPESP: 2019/00234-8 Swedish Research Council: 2016-01381 Swedish Research Council: 2020-01645 Swedish Heart and Lung Foundation: 20170124 Swedish Heart and Lung Foundation: 20180568 NovoNordisk: 0055026 |
| Databáze: | OpenAIRE |
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