Impaired Anticoagulant Effect of Heparin in the Artificial Kidney: An Experimental Study
| Autor: | H. C. Godal, J. Björnson |
|---|---|
| Rok vydání: | 1976 |
| Předmět: |
medicine.medical_specialty
Time Factors medicine.drug_class medicine.medical_treatment Clinical Biochemistry Acetates In Vitro Techniques Artificial kidney Models Biological Fibrin Internal medicine medicine Humans Magnesium Blood Coagulation Dialysis biology Heparin Chemistry Anticoagulant Batroxobin Proteolytic enzymes General Medicine Endocrinology Biochemistry Clotting time Prothrombin Time biology.protein Calcium Kidneys Artificial medicine.drug Discovery and development of direct thrombin inhibitors |
| Zdroj: | Scandinavian Journal of Clinical and Laboratory Investigation. 36:581-589 |
| ISSN: | 1502-7686 0036-5513 |
| DOI: | 10.1080/00365517609054482 |
| Popis: | Dialysis of blood and plasma was performed in vitro, in a 'mini-Kill' dialyser as well as in dialysis bags. A marked shortening of the thrombin-clotting time was observed, indicating fall in heparin anticoagulant effect. The concentration of heparin, however, as measured by polybrene titration, was substantially less reduced. Fibrin formation, as evidenced by the ethanol gelation test, occurred more often in the dialysed than in the control plasma. In conclusion, the discrepancy between concentration and anticoagulant effect of heparin could be partly explained by influx from the dialysate of calcium, magnesium, and acetate ions. The fibrin-polymerizing effect of these ions was confirmed by a shortening of the clotting time with Reptilase, a proteolytic enzyme not influenced by thrombin inhibitors such as heparin. In addition, liberation of platelet factor 4 may be responsible for some reduction in antithrombin activity of heparin. No evidence of heparin being dialysed or adhering to the dialysis membrane was found. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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