Antigen levels of the urokinase-type plasminogen activator and its gene polymorphisms in colorectal cancer
| Autor: | Karolina Przybyłowska, Janusz Blasiak, K Smolarczyk, Hanna Romanowicz-Makowska, Ulanska J, Pander B, A Dziki, Andrzej Kulig |
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| Rok vydání: | 2002 |
| Předmět: |
Male
Cancer Research Genotype Biology Metastasis Exon Gene Frequency Antigen medicine Humans Antigens Aged Urokinase Polymorphism Genetic Cancer Middle Aged medicine.disease Urokinase-Type Plasminogen Activator Oncology Cancer research Female Gene polymorphism Colorectal Neoplasms Plasminogen activator medicine.drug |
| Zdroj: | Cancer Letters. 181:23-30 |
| ISSN: | 0304-3835 |
| DOI: | 10.1016/s0304-3835(02)00038-1 |
| Popis: | We analysed the distribution of genotypes of two polymorphisms in the urokinase-type plasminogen activator (uPA) gene: C→T substitution in exon 6 and T→C substitution in intron 7 in 52 subjects with colorectal cancer. Genotypes were determined in tumour tissue and distant mucosa samples by allele-specific polymerase chain reaction. The antigen levels of uPA in cancer tissue were higher than in distant mucosa as measured by enzyme-linked immunosorbent assay. The level of uPA antigens in cancer samples with the C/C genotype of C→T polymorphism in exon 6 was higher than in samples with C/T and T/T genotypes. No differences in the level of uPA antigens between the alleles of the intron 7 T→C polymorphism were found. As uPA can be involved in cancer invasion and metastasis, C/C genotype in exon 6 of uPA gene can be further considered as being related to colorectal cancer progression. |
| Databáze: | OpenAIRE |
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