Molecular cloning of estrogen receptor alpha (ERα; ESR1) of the Japanese giant salamander, Andrias japonicus
| Autor: | Taisen Iguchi, Noriaki Santo, Hiroshi Urushitani, Louis J. Guillette, Tomohiro Oka, Shinji Minami, Yoshinao Katsu, Satomi Kohno, Naoko Mitsui, Kazuhide Ashikaga, Yukio Fukumoto, Kazushi Kuwabara, Shigeaki Kato, Yasuhiko Ohta, Osamu Tooi |
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| Rok vydání: | 2006 |
| Předmět: |
Transcriptional Activation
medicine.drug_class Molecular Sequence Data Urodela Estrogen receptor Japanese giant salamander CHO Cells Biology Transfection Biochemistry Estrogen-related receptor alpha Endocrinology Cricetinae Complementary DNA medicine Animals Tissue Distribution Amino Acid Sequence Cloning Molecular Molecular Biology Phylogeny Estrogen receptor beta Base Sequence Sequence Homology Amino Acid Estrogen Receptor alpha biology.organism_classification Molecular biology Protein Structure Tertiary Estrogen Female Estrogen-related receptor gamma Estrogen receptor alpha hormones hormone substitutes and hormone antagonists |
| Zdroj: | Molecular and Cellular Endocrinology. :84-94 |
| ISSN: | 0303-7207 |
| DOI: | 10.1016/j.mce.2006.07.001 |
| Popis: | Estrogens are essential for normal reproductive activity in females and males and for ovarian differentiation during a critical developmental stage in many vertebrates. To understand the molecular mechanisms of estrogen action and to evaluate estrogen receptor ligand interactions in the Japanese giant salamander ( Andrias japonicus ), we isolated cDNA encoding the estrogen receptor (ER) from the liver. A full-length Japanese giant salamander ER cDNA (jgsER) was obtained using 5′ and 3′ rapid amplification cDNA ends (RACE). The deduced amino acid sequence of the jgsER showed high identity to the Xenopus ERα (ESR1) (77.7%). We have applied both the conventional ERE-luciferase reporter assay system and the GAL4-transactivation system to characterize this receptor. In two different transient transfection assay systems using mammalian cells, the jgsER protein displayed estrogen-dependent activation of transcription. The GAL4-transactivation system showed about 10-fold greater activity of the estrogen receptor by hormone when compared to the conventional ERE-luciferase reporter assay system. Tissue distribution of ERα mRNA was examined and kidney, ovary and liver exhibited expression. This is the first isolation of an estrogen receptor from a salamander and also is the first functional cDNA obtained from the Japanese giant salamander, an endangered species considered a special natural monument of Japan. |
| Databáze: | OpenAIRE |
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