Combining electron crystallography and X-ray crystallography to study the MlotiK1 cyclic nucleotide-regulated potassium channel

Autor:	Gina M. Clayton, Jimin Wang, João H. Morais-Cabral, Steve G. Aller, Vinzenz M. Unger
Rok vydání:	2009
Předmět:	Models Molecular Crystallography Potassium Channels Chemistry Electron crystallography Protein Conformation Electrons Electron Weak interaction Crystallography X-Ray Negative stain Potassium channel Article Protein structure Structural Biology X-ray crystallography Projection (set theory)
Zdroj:	Journal of structural biology. 167(3)
ISSN:	1095-8657
Popis:	We have recently reported the X-ray structure of the cyclic nucleotide regulated potassium channel, MlotiK1. Here we describe the application of both electron and X-ray crystallography to obtain high quality crystals. We suggest that the combined application of these techniques provides a useful strategy for membrane protein structure determination. We also present negative stain projection and cryo-data projection maps. These maps provide new insights about the properties of the MlotiK1 channel. In particular, a comparison of a 9 Å cryo-data projection with calculated model maps strongly suggests that there is a very weak interaction between the pore and the S1-S4 domains of this 6 TM tetrameric cation channel and that the S1-S4 domains can adopt multiple orientations relative to the pore.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3156e251d4e8a6099958196a97072c5f https://pubmed.ncbi.nlm.nih.gov/19545635 Zobrazit plný text záznamu Full Text from ScienceDirect