Localization and partial characterization of thermostable glucoamylase produced by newly isolated Thermomyces lanuginosus TO3 in submerged fermentation
| Autor: | Eleni Gomes, Roberto da Silva, Ana Flávia Azevedo Carvalho, Aline Zorzetto Lopes Gonçalves |
|---|---|
| Přispěvatelé: | Universidade Estadual Paulista (Unesp) |
| Předmět: |
chemistry.chemical_classification
Multidisciplinary biology Starch Thermophile lcsh:Biotechnology Aspergillus niger submerged fermentation Substrate (chemistry) Maltose biology.organism_classification chemistry.chemical_compound Enzyme chemistry Biochemistry Thermomyces lanuginosus Glucoamylase lcsh:TP248.13-248.65 Fermentation Food science Thermostability |
| Zdroj: | ResearcherID Brazilian Archives of Biology and Technology v.51 n.4 2008 Brazilian Archives of Biology and Technology Instituto de Tecnologia do Paraná (Tecpar) instacron:TECPAR Brazilian Archives of Biology and Technology, Vol 51, Iss 4, Pp 657-665 (2008) Brazilian Archives of Biology and Technology, Volume: 51, Issue: 4, Pages: 657-665, Published: AUG 2008 SciELO Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
| ISSN: | 1516-8913 |
| Popis: | Submitted by Guilherme Lemeszenski (guilherme@nead.unesp.br) on 2013-08-22T18:40:55Z No. of bitstreams: 1 S1516-89132008000400024.pdf: 88171 bytes, checksum: d52a365b5a137e730bd7982db637e828 (MD5) Made available in DSpace on 2013-08-22T18:40:55Z (GMT). No. of bitstreams: 1 S1516-89132008000400024.pdf: 88171 bytes, checksum: d52a365b5a137e730bd7982db637e828 (MD5) Previous issue date: 2008-08-01 Made available in DSpace on 2013-09-30T19:27:07Z (GMT). No. of bitstreams: 2 S1516-89132008000400024.pdf: 88171 bytes, checksum: d52a365b5a137e730bd7982db637e828 (MD5) S1516-89132008000400024.pdf.txt: 30114 bytes, checksum: fb09951396460bac2c2a0229bcdab2a4 (MD5) Previous issue date: 2008-08-01 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T14:00:39Z No. of bitstreams: 2 S1516-89132008000400024.pdf: 88171 bytes, checksum: d52a365b5a137e730bd7982db637e828 (MD5) S1516-89132008000400024.pdf.txt: 30114 bytes, checksum: fb09951396460bac2c2a0229bcdab2a4 (MD5) Made available in DSpace on 2014-05-20T14:00:39Z (GMT). No. of bitstreams: 2 S1516-89132008000400024.pdf: 88171 bytes, checksum: d52a365b5a137e730bd7982db637e828 (MD5) S1516-89132008000400024.pdf.txt: 30114 bytes, checksum: fb09951396460bac2c2a0229bcdab2a4 (MD5) Previous issue date: 2008-08-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) O fungo termofílico Thermomyces lanuginosus TO3 foi isolado a partir de amostras de material de pilhas de compostagem, com base em sua capacidade de crescer em meio líquido contendo amido como única fonte de carbono, a 45 ºC, e produzir considerável quantidade de glucoamilase. A produção da enzima por fermentação submersa FSm foi alta, com um máximo de 13 U/mL em 168 h de fermentação. A atividade enzimática foi maior sobre amido do que sobre a maltose e maltooligosacarideos. As atividades de glucoamilase extra e intracelular variaram com o tempo de fermentação. A glucoamilase produzidas por T. lanuginosus TO3 apresentou elevada temperatura ótima de atividade (65 -70 ºC) com boa termoestabilidade em ausência de substrato, apresentando uma meia vida de 6 h a 60ºC, além de estabilidade em ampla faixa de pH. Os resultados apresentados indicam uma importante fonte alternativa de glucoamilase para uso no processamento industrial de amido. Thermophilic Thermomyces lanuginosus strain TO3 was isolated from compost pile samples and was used for its ability to produce considerable glucoamylase activity when growing in liquid medium at 45ºC with starch as the sole carbon source. Enzyme productivity was high in submerged fermentation (SmF) with maximum activity of 13 U/mL after 168 h of fermentation. Higher quantities of glucose were released when the substrate for enzyme was soluble starch than maltose or maltooligosaccharides were used. The distribution of glucoamylase between the extracellular and cell-associated fractions varied according to fermentation time. Glucoamylase produced from T. lanuginosus TO3 had optimum activity at 65 ºC and good thermostability in the absence of substrate, with a half-life of 6 h at 60 ºC. The enzyme was stable over a wide pH range (4.0-10.0). UNESP Instituto de Biociências Letras e Ciências Exatas Departamento de Biologia UNESP Instituto de Biociências Letras e Ciências Exatas Departamento de Biologia |
| Databáze: | OpenAIRE |
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