Differentiation of Campylobacter species by protein banding patterns in polyacrylamide slab gels

Autor: D W Lambe, D A Ferguson
Rok vydání: 1984
Předmět:
Zdroj: Journal of Clinical Microbiology. 20:453-460
ISSN: 1098-660X
0095-1137
DOI: 10.1128/jcm.20.3.453-460.1984
Popis: Soluble protein extracts of 37 catalase-positive strains of Campylobacter species were examined by polyacrylamide slab gel electrophoresis (PAGE). Electrophoretic banding patterns showed good correlation with biochemical tests and with available DNA homology data in distinguishing species of Campylobacter but did not differentiate subspecies or biotypes. PAGE patterns indicated that Campylobacter coli is a distinct species. Furthermore, the PAGE patterns indicated that C. jejuni and nalidixic acid-resistant thermophilic Campylobacter species (C. laridis) are each distinct species. The protein banding patterns of C. fetus subsp. venerealis and C. fetus subsp. fetus strains were distinctly different from those of the three thermophilic species.
Databáze: OpenAIRE