The nucleotide sequence of fragment HindIII-C of human adenovirus type 5 DNA (map positions 17.1-31.7)
| Autor: | H. van Ormondt, B.M.M. Dekker |
|---|---|
| Rok vydání: | 1984 |
| Předmět: |
Genetics
biology Base Sequence DNA polymerase viruses Adenoviruses Human Nucleic acid sequence Protein primary structure General Medicine DNA Restriction Enzymes Molecular biology DNA sequencing Restriction fragment chemistry.chemical_compound chemistry Transcription (biology) DNA Viral biology.protein RNA Viral RNA Messenger Codon DNA Polymerase |
| Zdroj: | Gene. 27(1) |
| ISSN: | 0378-1119 |
| Popis: | This paper describes the sequence of nucleotides 6246–11570 between the two Hin dIII sites at map positions (m.p.) 17.1 and 31.7 of human adenovirus serotype 5 (Ad5) DNA. It is 99% homologous with the corresponding Hin dIII-B fragment of adenovirus type 2 (Ad2; Gingeras et al., 1982; Alestrom et al., 1982) which has been shown to encode the second, “i”, and third leaders of the major late RNAs, the virus-associated RNAs VA I and VA II (all rightward transcripts), and in the opposite sense the early-region E2b mRNAs for a DNA polymerase and for the terminal protein precursor (pTP). Except for the latter, it was possible to determine the coordinates of the corresponding Ad5 RNAs, either by sequence homology with Ad2, or by nuclease S1 analysis. The Ad5 DNA sequence contains the same open reading frames as that of Ad2. The longest of these lie in the r -strand and would specify proteins of M r 120377 (DNA polymerase) and 74558 (pTP). |
| Databáze: | OpenAIRE |
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