Salmonella Typhimurium utilizes a T6SS-mediated antibacterial weapon to establish in the host gut
| Autor: | Denise M. Monack, Eric Cascales, Thibault G. Sana, Nicolas Flaugnatti, Virginie Baylot, Lilian H. Lam, Laure Journet, Amanda Jacobson, Kyler A. Lugo, Eric Durand |
|---|---|
| Přispěvatelé: | Stanford University, Laboratoire d'ingénierie des systèmes macromoléculaires (LISM), Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Aix Marseille Université (AMU) |
| Rok vydání: | 2016 |
| Předmět: |
Male
Salmonella typhimurium 0301 basic medicine Salmonella [SDV]Life Sciences [q-bio] Gut flora medicine.disease_cause Mice Pathogen ComputingMilieux_MISCELLANEOUS anti-prokaryotic activity Multidisciplinary [SDV.BBM.BS]Life Sciences [q-bio]/Biochemistry Molecular Biology/Structural Biology [q-bio.BM] biology Effector Klebsiella oxytoca toxins Type VI Secretion Systems T6SS [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology PNAS Plus Salmonella enterica Female Genomic Islands Virulence Factors 030106 microbiology [SDV.BC]Life Sciences [q-bio]/Cellular Biology Tae4 amidase digestive system Microbiology Bile Acids and Salts 03 medical and health sciences Bacterial Proteins Antibiosis gut colonization microbiota medicine Animals [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry Molecular Biology/Biochemistry [q-bio.BM] Type VI secretion system Salmonella Infections Animal Type VI Secretion System SPI-6 [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry Molecular Biology/Molecular biology biology.organism_classification Culture Media Gastrointestinal Microbiome Mice Inbred C57BL gastrointestinal tract Bacteria |
| Zdroj: | Proceedings of the National Academy of Sciences of the United States of America Proceedings of the National Academy of Sciences of the United States of America, 2016, 113 (34), pp.E5044-E5051. ⟨10.1073/pnas.1608858113⟩ Proceedings of the National Academy of Sciences of the United States of America, National Academy of Sciences, 2016, 113 (34), pp.E5044-E5051. ⟨10.1073/pnas.1608858113⟩ |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.1608858113 |
| Popis: | The mammalian gastrointestinal tract is colonized by a high-density polymicrobial community where bacteria compete for niches and resources. One key competition strategy includes cell contact-dependent mechanisms of interbacterial antagonism, such as the type VI secretion system (T6SS), a multiprotein needle-like apparatus that injects effector proteins into prokaryotic and/or eukaryotic target cells. However, the contribution of T6SS antibacterial activity during pathogen invasion of the gut has not been demonstrated. We report that successful establishment in the gut by the enteropathogenic bacterium Salmonella enterica serovar Typhimurium requires a T6SS encoded within Salmonella pathogenicity island-6 (SPI-6). In an in vitro setting, we demonstrate that bile salts increase SPI-6 antibacterial activity and that S. Typhimurium kills commensal bacteria in a T6SS-dependent manner. Furthermore, we provide evidence that one of the two T6SS nanotube subunits, Hcp1, is required for killing Klebsiella oxytoca in vitro and that this activity is mediated by the specific interaction of Hcp1 with the antibacterial amidase Tae4. Finally, we show that K. oxytoca is killed in the host gut in an Hcp1-dependent manner and that the T6SS antibacterial activity is essential for Salmonella to establish infection within the host gut. Our findings provide an example of pathogen T6SS-dependent killing of commensal bacteria as a mechanism to successfully colonize the host gut. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|