Microbiology and biofilm of corneal sutures
| Autor: | Richard K. Forster, Oriel Spierer, Oded Rock, Eldad Adler, Darlene Miller |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
medicine.medical_specialty
Bacterial growth Eye Infections Bacterial Cornea 03 medical and health sciences Cellular and Molecular Neuroscience 0302 clinical medicine Suture (anatomy) Ophthalmology medicine Bacteriology Humans 030212 general & internal medicine Prospective Studies Keratitis Bacteriological Techniques Bacteria Sutures business.industry Biofilm Sensory Systems medicine.anatomical_structure Biofilms 030221 ophthalmology & optometry Microscopy Electron Scanning Positive culture business Retention time Keratoplasty Penetrating |
| Zdroj: | The British journal of ophthalmology. 102(11) |
| ISSN: | 1468-2079 |
| Popis: | AimTo investigate the relationships between corneal suture bacteriology, biofilm and the clinical setting using culture studies and scanning electron microscopy (SEM).MethodsThis is a prospective, observational study of patients with a history of penetrating keratoplasty presenting to a tertiary cornea clinic for routine or symptoms-related corneal suture removal. We documented for each patient the suture clinical setting (quiescent, exposed and keratitis-related), retention time, antimicrobial therapy, bacterial growth on culture studies, and bacterial presence and biofilm coverage on SEM.ResultsThere were significantly different culture positivity rates between the quiescent (8%), exposed (12%) and keratitis-related (60%) suture groups (p=0.039). As expected, keratitis-related sutures had the longest retention time compared with quiescent and exposed ones (p=0.02). The biofilm coverage score was higher for sutures from the keratitis-related and exposed groups, although this trend was not statistically significant (p=0.90). Higher biofilm scores were seen in samples that also yielded a positive culture result (p=0.36) and in samples with bacterial presence on SEM images (p=0.16 and p=0.73). Both of these were important trends but not statistically significant.ConclusionsEvidence for active bacterial and biofilm presence on corneal sutures was found. Corneal sutures should be considered for removal sooner, before becoming exposed and/or keratitis-related. Traditional culture studies and SEM imaging are helpful in investigating biofilm and its clinical importance. More studies of the spectrum of bacterial growth on embedded biomedical devices such as corneal sutures are needed. |
| Databáze: | OpenAIRE |
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