Deciphering sex-specific miRNAs as heat-recorders in zebrafish
| Autor: | T.A. van Gelderen, J. Montfort, J.A. Álvarez-Dios, V. Thermes, F. Piferrer, J. Bobe, L. Ribas |
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| Přispěvatelé: | Ministerio de Ciencia, Innovación y Universidades (España), Ministerio de Economía y Competitividad (España), Agencia Estatal de Investigación (España) |
| Rok vydání: | 2021 |
| Předmět: |
Male
Adult Marine biology Multidisciplinary Hot Temperature Sex Differentiation Gene Expression Profiling Reproductive biology Non-coding RNAs Environmental impact MicroRNAs Animals Humans Female Gonads Conserve and sustainably use the oceans seas and marine resources for sustainable development Zebrafish Biomarkers |
| DOI: | 10.1101/2021.12.29.474410 |
| Popis: | 14 pages, 7 figures, supplementary information https://doi.org/10.1038/s41598-022-21864-3.-- Data availability: The datasets generated and analyzed during the current study are available in NCBI SRA repository with the accession number: PRJNA755482 In the last decade, a plethora of microRNAs (miRNAs) has been reported in a wide variety of physiological processes, including reproduction, in many aquatic organisms. However, miRNAome alterations occurred by environmental cues due to water temperature increment have not yet been elucidated. With the aim to identify epigenetic regulations mediated by miRNAs in the gonads in a climate change scenario, the animal model zebrafish (Danio rerio) were subjected to high temperatures during sex differentiation, a treatment that results in male-skewed sex ratios in the adulthood. Once the fish reached adulthood, gonads were sequenced by high-throughput technologies and a total of 23 and 1 differentially expressed miRNAs in ovaries and testes, respectively, were identified two months after the heat treatment. Most of these heat-recorder miRNAs were involved in human sex-related cancer and about 400 predicted-target genes were obtained, some with reproduction-related functions. Their synteny in the zebrafish genome was, for more than half of the predicted target genes, in the chromosomes 7, 2, 4, 3 and 11 in the ovaries, chromosome 4 being the place where the sex-associated-region (sar) is localized in wild zebrafish. Further, spatial localization in the gonads of two selected heat-recorder miRNAs (miR-122-5p and miR-146-5p) showed exclusive expression in the ovarian germ cells. The present study expands the catalog of sex-specific miRNAs and deciphers, for the first time, thermosensitive miRNAs in the zebrafish gonads that might be used as potential epimarkers to predict environmental past events This study was supported by the Spanish Ministry of Science and Innovation grant AGL2015-73864-JIN “Ambisex” and 2PID2020-113781RB-I00 “MicroMet” and by the Consejo Superior de Investigaciones Científicas (CSIC) grant 02030E004 “Interomics” to LR, by grant AEI grant PID2019-108888RB-I00 to FP and with funding from the Spanish government through the ‘Severo Ochoa Centre of Excellence’ accreditation (CEX2019-000928-S) |
| Databáze: | OpenAIRE |
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