Interleukin-6 gene expression and production induced in human monocytes by membrane proteoglycans from Klebsiella pneumoniae
| Autor: | Marina Sironi, Antonio Sica, Alberto Mantovani, Luciano Licciardello, Francesco Colotta, F. Riganti |
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| Rok vydání: | 1990 |
| Předmět: |
Lipopolysaccharides
Immunology Gene Expression In Vitro Techniques Biology Monocytes Microbiology Gene product Gene expression medicine Humans RNA Messenger Northern blot Pharmacology Interleukin-6 Monocyte Interleukin Blotting Northern Molecular biology Recombinant Proteins In vitro Blot Klebsiella pneumoniae medicine.anatomical_structure Cell culture Proteoglycans Bacterial Outer Membrane Proteins |
| Zdroj: | International Journal of Immunopharmacology. 12:397-402 |
| ISSN: | 0192-0561 |
| DOI: | 10.1016/0192-0561(90)90021-e |
| Popis: | The present study was designed to investigate the effect of membrane proteoglycans (MPG) from Klebsiella pneumoniae on IL-6 production by human peripheral blood monocytes. Exposure in vitro to MPG induced release of IL-6 activity from human monocytes, as assessed by the 7TD1 hybridoma assay. MPG-induced hybridoma growth factor activity was blocked by anti-IL-6 antibodies. MPG induced expression in human monocytes of IL-6 mRNA transcripts as assessed by Northern blot analysis. Induction of IL-6 in mononuclear phagocytes may play a role in the immunomodulatory activity of MPG. |
| Databáze: | OpenAIRE |
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