Phospholipid Membrane Protection by Sugar Molecules during Dehydration—Insights into Molecular Mechanisms Using Scattering Techniques
| Autor: | Ben Kent, Gary Bryant, Christopher J. Garvey, Karen L. Koster, Thomas Lenné |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
0106 biological sciences
Membrane lipids Phospholipid Carbohydrates Review Neutron scattering contrast variation small angle neutron scattering 01 natural sciences Models Biological Catalysis Inorganic Chemistry lcsh:Chemistry 03 medical and health sciences chemistry.chemical_compound Membrane Lipids X-Ray Diffraction anhydrobiology Scattering Small Angle cryobiology Physical and Theoretical Chemistry Molecular Biology lcsh:QH301-705.5 Spectroscopy phospholipids 030304 developmental biology 0303 health sciences Dehydration Scattering Chemistry Small-angle X-ray scattering Organic Chemistry Cell Membrane Membrane structure General Medicine X-ray scattering Small-angle neutron scattering Computer Science Applications Crystallography Neutron Diffraction Membrane sugars lcsh:Biology (General) lcsh:QD1-999 Chemical physics membranes Carbohydrate Metabolism lipids (amino acids peptides and proteins) Synchrotrons 010606 plant biology & botany |
| Zdroj: | International Journal of Molecular Sciences, Vol 14, Iss 4, Pp 8148-8163 (2013) International Journal of Molecular Sciences |
| ISSN: | 1422-0067 |
| Popis: | Scattering techniques have played a key role in our understanding of the structure and function of phospholipid membranes. These techniques have been applied widely to study how different molecules (e.g., cholesterol) can affect phospholipid membrane structure. However, there has been much less attention paid to the effects of molecules that remain in the aqueous phase. One important example is the role played by small solutes, particularly sugars, in protecting phospholipid membranes during drying or slow freezing. In this paper, we present new results and a general methodology, which illustrate how contrast variation small angle neutron scattering (SANS) and synchrotron-based X-ray scattering (small angle (SAXS) and wide angle (WAXS)) can be used to quantitatively understand the interactions between solutes and phospholipids. Specifically, we show the assignment of lipid phases with synchrotron SAXS and explain how SANS reveals the exclusion of sugars from the aqueous region in the particular example of hexagonal II phases formed by phospholipids. |
| Databáze: | OpenAIRE |
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