Lymphatic vessel density, microvessel density and lymphangiogenic growth factor expression in colorectal cancer

Autor: Shant Kumar, Maria Jeziorska, David J Sherlock, Najib Haboubi, Gordon C Jayson, Sarah E Duff, Sarah T O'Dwyer
Rok vydání: 2007
Předmět:
Zdroj: Colorectal Disease. 9:793-800
ISSN: 1463-1318
1462-8910
DOI: 10.1111/j.1463-1318.2006.01199.x
Popis: OBJECTIVE: Microvessel density (MVD) has been studied as a prognostic marker in human cancers. Quantification of lymphatic vessel density (LVD) is now possible by using new antibodies. Expression of the lymphangiogenic growth factors, VEGF-C and VEGF-D, is associated with poorer clinicopathological outcomes in various tumours. The aim of this study was to quantify LVD and MVD in colorectal cancer, determine the relationship between LVD, MVD and clinicopathological variables and examine the relationship between LVD and tumour expression of VEGF-C and VEGF-D. METHOD: Thirty primary colorectal cancers were immunostained for CD34, lymph vessel endothelial hyaluronan receptor-1 (LYVE-1), VEGF-A and VEGF-D using standard techniques. LVD and MVD were determined by Chalkley grid counting. Tumours were assessed for the presence or absence of LYVE-1 positive lymphatics at different areas within the tumour and the tumour was scored for VEGF-C and VEGF-D immunostaining intensity at the invading tumour edge. Non-parametric tests were used for statistical analysis and a P-value of
Databáze: OpenAIRE
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