Production of live foals via intracytoplasmic injection of lyophilized sperm and sperm extract in the horse
| Autor: | Katrin Hinrichs, Charles C. Love, David L. Hartman, Young Ho Choi, Dickson D. Varner |
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| Rok vydání: | 2011 |
| Předmět: |
Male
endocrine system Embryology Cell Survival Embryonic Development Semen DNA Fragmentation Biology Andrology Endocrinology Human fertilization Pregnancy biology.animal medicine Animals Horses Sperm Injections Intracytoplasmic Blastocyst reproductive and urinary physiology urogenital system Pregnancy Outcome Obstetrics and Gynecology Horse Embryo Cell Biology Embryo Mammalian Sperm Freeze Drying medicine.anatomical_structure Reproductive Medicine Foal Pregnancy Animal DNA fragmentation Female Live Birth |
| Zdroj: | REPRODUCTION. 142:529-538 |
| ISSN: | 1741-7899 1470-1626 |
| DOI: | 10.1530/rep-11-0145 |
| Popis: | Work with lyophilized sperm helps delineate the factors required for successful fertilization. We investigated the use of lyophilized sperm in equine embryo production. In Experiment 1, sperm DNA fragmentation index was not affected by three freeze/thaw or lyophilization cycles. In Experiment 2, oocytes injected with lyophilized sperm or with sperm from a treatment in which lyophilized sperm were suspended in sperm cytoplasmic extract (SE) yielded blastocyst development rates of 0 and 28% respectively (P |
| Databáze: | OpenAIRE |
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