Normal-phase thin-layer chromatography of some angiotensin converting enzyme (ACE) inhibitors and their metabolites
| Autor: | Zivoslav Tesic, Biljana Stojimirovic, Mirjana Aleksic, Jadranka Odović, Dušanka Milojković-Opsenica |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
Solvent system
chemistry.chemical_classification Chromatography ACE inhibitors biology Normal phase 010401 analytical chemistry normal-phase thin-layer chromatography Angiotensin-converting enzyme General Chemistry 010402 general chemistry 01 natural sciences Thin-layer chromatography 0104 chemical sciences Partition coefficient lcsh:Chemistry Enzyme Linear relationship chemistry lcsh:QD1-999 biology.protein Active metabolite hydrophobicity |
| Zdroj: | Journal of the Serbian Chemical Society, Vol 74, Iss 6, Pp 677-688 (2009) Journal of the Serbian Chemical Society |
| ISSN: | 0352-5139 |
| Popis: | The separation and chromatographic behaviour of five ACE (angiotensin converting enzyme) inhibitors and their four active metabolites were investigated by normal-phase thin-layer chromatography on silica using several mono- and binary non-aqueous solvent systems. The linear relationship between the R(M) values and the composition 0 of employed mobile phase was obtained. The hydrophobicity parameters R(M)(0) and C(0) were determined from the regression data of the plots, analogous to reversed-phase chromatography. The chromatographically obtained hydrophobicity parameters were correlated with the calculated log P values. The current results were correlated with the lipophilicity of the studied ACE inhibitors and their metabolites, previously estimated by reversed-phase chromatography. Hromatografsko razdvajanje I ponašanje pet ASE inhibitora (inhibitora angio-tenzin-konvertujućeg enzima) I njihova četiri aktivna metabolita ispitivano je metodom normalno-fazne tankoslojne hromatografije na silika-gelu primenom nekoliko jedno- I dvo-komponentnih nevodenih sistema rastvarača. Dobijena je linearna zavisnost između RM-vrednosti I koncentracije etanola u mobilnoj fazi. Na osnovu odgovarajućih regresionih podataka, po analogiji sa reverzno-faznom hromatografijom, izračunati su parametri lipofilnosti 0M R I C0. Hromatografski dobijeni parametri hidrofobnosti korelisani su sa izračunatim log P vrednostima. Takođe, 0M R -vrednosti dobijene u ovom radu korelisane su sa 0M R -vrednostima dobijenim metodom reverzno-fazne tankoslojne hromatografije. |
| Databáze: | OpenAIRE |
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