Interleukin-4, -5, and -6 and tumor necrosis factor-alpha in normal and asthmatic airways: evidence for the human mast cell as a source of these cytokines
| Autor: | June Roberts, C H Heusser, KM Britten, R Mueller, Stephen Montefort, Peter H. Howarth, Ratko Djukanovic, Stephen T. Holgate, Peter Bradding |
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| Rok vydání: | 1994 |
| Předmět: |
Adult
Male Pulmonary and Respiratory Medicine Pathology medicine.medical_specialty medicine.medical_treatment Clinical Biochemistry Bronchi Tryptase Immune system medicine Humans Mast Cells Molecular Biology Interleukin 4 Mucous Membrane biology Tumor Necrosis Factor-alpha business.industry Interleukins Interleukin Cell Biology Mast cell Immunohistochemistry Asthma Eosinophils Interleukin 33 Cytokine medicine.anatomical_structure Immunology biology.protein Female Tumor necrosis factor alpha business |
| Zdroj: | American Journal of Respiratory Cell and Molecular Biology. 10:471-480 |
| ISSN: | 1535-4989 1044-1549 |
| DOI: | 10.1165/ajrcmb.10.5.8179909 |
| Popis: | Asthma is characterized by the presence of an inflammatory cell infiltrate in the bronchial mucosa consisting of activated mast cells, eosinophils, and T cells. Several cytokines are considered to play a pivotal role in this response, particularly interleukin (IL)-4, IL-5, IL-6, and tumor necrosis factor-alpha (TNF-alpha). In this study, we have used immunohistochemistry applied to thin glycol methacrylate sections of bronchial mucosal biopsies to define the cellular provenance of these cytokines in normal and asthmatic airways. Both the asthmatic and normal mucosa contained numerous cells staining positively for all four cytokines, with the majority identified as mast cells by their tryptase content. Eosinophils also accounted for some IL-5 immunostaining in the asthmatic biopsies. By using two monoclonal antibodies directed to different epitopes of IL-4, we provide tentative evidence for enhanced IL-4 secretion in asthma. Similarly, a sevenfold increase in the number of mast cells staining for TNF-alpha in the asthmatic biopsies suggests that this cytokine is also up-regulated in this disease. These findings clearly identify human mast cells as a source of IL-4, IL-5, IL-6, and TNF-alpha and add to the view that, along with T cells, mast cells may play an important role in initiating and maintaining the inflammatory response in asthma. |
| Databáze: | OpenAIRE |
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