BAG3 regulates total MAP1LC3B protein levels through a translational but not transcriptional mechanism
| Autor: | Daniel Peña-Oyarzun, Andrea E. Rodriguez, Camila López-Crisosto, Clara Quiroga, Tobias Morawe, Valentina Parra, Daniela Salas, Sergio Lavandero, Mario Chiong, Christian Behl |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Proteasome Endopeptidase Complex Transcription Genetic ATG8 ATG5 Biology BAG3 ATG12 03 medical and health sciences 0302 clinical medicine Protein biosynthesis Humans RNA Messenger Molecular Biology Adaptor Proteins Signal Transducing Genetics Gene knockdown Autophagy Cell Biology Lipids Basic Research Paper Cell biology HEK293 Cells 030104 developmental biology Protein Biosynthesis Proteolysis Apoptosis Regulatory Proteins Lysosomes Microtubule-Associated Proteins MAP1LC3B 030217 neurology & neurosurgery HeLa Cells |
| Zdroj: | Autophagy Artículos CONICYT CONICYT Chile instacron:CONICYT Europe PubMed Central |
| Popis: | Autophagy is mainly regulated by post-translational and lipid modifications of ATG proteins. In some scenarios, the induction of autophagy is accompanied by increased levels of certain ATG mRNAs such as MAP1LC3B/LC3B, ATG5 or ATG12. However, little is known about the regulation of ATG protein synthesis at the translational level. The cochaperone of the HSP70 system BAG3 (BCL2-associated athanogene 3) has been associated to LC3B lipidation through an unknown mechanism. In the present work, we studied how BAG3 controls autophagy in HeLa and HEK293 cells. Our results showed that BAG3 regulates the basal amount of total cellular LC3B protein by controlling its mRNA translation. This effect was apparently specific to LC3B because other ATG protein levels were not affected. BAG3 knockdown did not affect LC3B lipidation induced by nutrient deprivation or proteasome inhibition. We concluded that BAG3 maintains the basal amount of LC3B protein by controlling the translation of its mRNA in HeLa and HEK293 cells. |
| Databáze: | OpenAIRE |
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