The complete genome sequence of the algal symbiont Dinoroseobacter shibae: a hitchhiker's guide to life in the sea
| Autor: | Amrita Pati, Ralf Rabus, Thorsten Brinkhoff, Daniela Kalhoefer, Wolfgang Liebl, Sarah Hahnke, Ina Buchholz, Rolf Daniel, Thomas Drepper, Hajnalka Kiss, Heiko Liesegang, Jörn Petersen, Claudia Pommerenke, Irene Wagner-Döbler, Heribert Cypionka, Boyke Bunk, Martine Berger, Petra Tielen, Cliff Han, Tanja Piekarski, Hans-Peter Klenk, Nittaya Wanphrut, Erko Stackebrandt, Linda S. Thompson, Antje Wichels, Dieter Jahn, Nikos C. Kyrpides, Britta Ballhausen, Meinhard Simon, Hajo Zech, Mathias von Jan, Silke Pradella, Sebastian Thole, Gunnar Gerdts, Linda Meincke, Rüdiger Pukall, Jürgen Tomasch |
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| Rok vydání: | 2009 |
| Předmět: |
DNA
Bacterial Aerobic bacteria Molecular Sequence Data Sequence Homology Synteny Microbiology 03 medical and health sciences Plasmid Gene cluster Dimethyl Sulfoxide Anaerobiosis Thiamine 14. Life underwater Rhodobacteraceae Symbiosis Gene Ecology Evolution Behavior and Systematics 030304 developmental biology Genetics 0303 health sciences Nitrates biology 030306 microbiology Eukaryota Sequence Analysis DNA Roseobacter biology.organism_classification Aerobiosis Biosynthetic Pathways Rhodobacterales Vitamin B 12 Regulon Horizontal gene transfer Genome Bacterial Plasmids |
| Zdroj: | The ISME Journal. 4:61-77 |
| ISSN: | 1751-7370 1751-7362 |
| DOI: | 10.1038/ismej.2009.94 |
| Popis: | Dinoroseobacter shibae DFL12(T), a member of the globally important marine Roseobacter clade, comprises symbionts of cosmopolitan marine microalgae, including toxic dinoflagellates. Its annotated 4 417 868 bp genome sequence revealed a possible advantage of this symbiosis for the algal host. D. shibae DFL12(T) is able to synthesize the vitamins B(1) and B(12) for which its host is auxotrophic. Two pathways for the de novo synthesis of vitamin B(12) are present, one requiring oxygen and the other an oxygen-independent pathway. The de novo synthesis of vitamin B(12) was confirmed to be functional, and D. shibae DFL12(T) was shown to provide the growth-limiting vitamins B(1) and B(12) to its dinoflagellate host. The Roseobacter clade has been considered to comprise obligate aerobic bacteria. However, D. shibae DFL12(T) is able to grow anaerobically using the alternative electron acceptors nitrate and dimethylsulfoxide; it has the arginine deiminase survival fermentation pathway and a complex oxygen-dependent Fnr (fumarate and nitrate reduction) regulon. Many of these traits are shared with other members of the Roseobacter clade. D. shibae DFL12(T) has five plasmids, showing examples for vertical recruitment of chromosomal genes (thiC) and horizontal gene transfer (cox genes, gene cluster of 47 kb) possibly by conjugation (vir gene cluster). The long-range (80%) synteny between two sister plasmids provides insights into the emergence of novel plasmids. D. shibae DFL12(T) shows the most complex viral defense system of all Rhodobacterales sequenced to date. |
| Databáze: | OpenAIRE |
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