A Method for the Preservation of Human Blood Group Erythrocyte Antigens in Liquid Nitrogen for a Test Cell Panel
| Autor: | H. S. Bowman, R. K. Cunningham, J. F. Mohn |
|---|---|
| Rok vydání: | 1970 |
| Předmět: |
Glycerol
Isoantigens Lysis Erythrocytes Nitrogen Biology Cryopreservation Andrology chemistry.chemical_compound Antigen Freezing Extracellular Methods Humans Sorbitol Hemagglutination Tests Hematology General Medicine Liquid nitrogen Stainless Steel Biochemistry chemistry Blood Preservation Blood Group Antigens Intracellular |
| Zdroj: | Vox Sanguinis. 19:508-521 |
| ISSN: | 1423-0410 0042-9007 |
| DOI: | 10.1159/000466040 |
| Popis: | A modification of Krijnen's cryopreservation method, using low intracellular concentrations of 17.5% glycerol and extracellular 4% sorbitol as additives, has been developed for liquid nitrogen freezing of small volumes of erythrocytes for a test cell panel. A technique for their subsequent successful storage in sterile, dextrose-electrolyte maintenance solution (CP-2) at 4°C after recovery is presented. By these procedures, the mean recovery of red cells after lytic losses from freezing and thawing injury was 94.1%. The manipulations involved in transferring, mixing and resuspending the red cells removed an additional 11.1–16.2%, resulting in an overall recovery of 80.5% for serologic tests. Less than 3% of the cells exhibited cumulative lysis at seven days maintenance in CP-2 solution at 4°C, and the blood group antigens examined remained as potent as those in sterile, fresh ACD blood. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text