Oxidative and nitrosative stress in frozen-thawed pig spermatozoa. II: Effect of the addition of saccharides to freezing medium on sperm function
| Autor: | Claudia Moya, Pamela Uribe, Felipe Pezo, Rafael A. Burgos, Marc Yeste, André Furugen Cesar de Andrade, Raúl Sánchez, Jennie Risopatrón, Fabiola Zambrano |
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| Rok vydání: | 2020 |
| Předmět: |
Male
Swine Motility General Biochemistry Genetics and Molecular Biology Cryopreservation Lipid peroxidation Andrology 03 medical and health sciences chemistry.chemical_compound Cryoprotective Agents 0302 clinical medicine Freezing Animals Viability assay Acrosome chemistry.chemical_classification Reactive oxygen species 030219 obstetrics & reproductive medicine Chemistry 0402 animal and dairy science 04 agricultural and veterinary sciences General Medicine Spermatozoa 040201 dairy & animal science Sperm Oxidative Stress Sperm Motility General Agricultural and Biological Sciences Peroxynitrite Semen Preservation |
| Zdroj: | Cryobiology. 97:5-11 |
| ISSN: | 0011-2240 |
| Popis: | Saccharides have bioprotective properties, with a high capacity to preserve biological proteins and membranes during sperm cryopreservation. The aim of this study was to evaluate how replacing the lactose of cryopreservation media by sucrose (SUC) or trehalose (TRE) at concentrations of 0.2 M (SUC-1 and TRE-1) and 0.25 M (SUC-2 and TRE-2) affects frozen/thawed pig spermatozoa. The media used were composed of medium A (saccharide/egg yolk) and B (saccharide/egg yolk/glycerol), their osmolality being determined prior to freezing. Cell viability, membrane lipid disorder, acrosome integrity, mitochondrial membrane potential (MMP), lipid peroxidation, thiol group oxidation, total reactive oxygen species (ROS), peroxynitrite and superoxide anion (O2●-) were determined through flow cytometry; total motility (TM), progressive motility (PM) and kinetic parameters motility were determined immediately after thawing (T0) and again 30 (T30) and 60 (T60) minutes later. The SUC-2 and TRE-2 groups maintained viability significantly and presented fewer lipid membrane disorders, respectively, both with a significant increase in MMP. The production of O2●- and peroxynitrite was lower in the TRE-2 groups compared to the control (P |
| Databáze: | OpenAIRE |
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