Scaffoldless tissue-engineered nerve conduit promotes peripheral nerve regeneration and functional recovery after tibial nerve injury in rats
Autor: | Jacob P Mertens, Tatiana Y. Kostrominova, Lisa M. Larkin, Keith W VanDusen, Aaron M. Adams |
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Rok vydání: | 2017 |
Předmět: |
0301 basic medicine
nerve regeneration peripheral nerve repair neural conduit tissue engineering fibroblasts neural cells Nerve guidance conduit lcsh:RC346-429 03 medical and health sciences 0302 clinical medicine Developmental Neuroscience Tissue engineering Medicine Tibial nerve lcsh:Neurology. Diseases of the nervous system Specific force business.industry Regeneration (biology) Histology Anatomy musculoskeletal system 030104 developmental biology Peripheral nerve injury Epineurial repair business 030217 neurology & neurosurgery Research Article |
Zdroj: | Neural Regeneration Research Neural Regeneration Research, Vol 12, Iss 9, Pp 1529-1537 (2017) |
ISSN: | 1673-5374 |
Popis: | Damage to peripheral nerve tissue may cause loss of function in both the nerve and the targeted muscles it innervates. This study compared the repair capability of engineered nerve conduit (ENC), engineered fibroblast conduit (EFC), and autograft in a 10-mm tibial nerve gap. ENCs were fabricated utilizing primary fibroblasts and the nerve cells of rats on embryonic day 15 (E15). EFCs were fabricated utilizing primary fibroblasts only. Following a 12-week recovery, nerve repair was assessed by measuring contractile properties in the medial gastrocnemius muscle, distal motor nerve conduction velocity in the lateral gastrocnemius, and histology of muscle and nerve. The autografts, ENCs and EFCs reestablished 96%, 87% and 84% of native distal motor nerve conduction velocity in the lateral gastrocnemius, 100%, 44% and 44% of native specific force of medical gastrocnemius, and 63%, 61% and 67% of native medial gastrocnemius mass, respectively. Histology of the repaired nerve revealed large axons in the autograft, larger but fewer axons in the ENC repair, and many smaller axons in the EFC repair. Muscle histology revealed similar muscle fiber cross-sectional areas among autograft, ENC and EFC repairs. In conclusion, both ENCs and EFCs promoted nerve regeneration in a 10-mm tibial nerve gap repair, suggesting that the E15 rat nerve cells may not be necessary for nerve regeneration, and EFC alone can suffice for peripheral nerve injury repair. |
Databáze: | OpenAIRE |
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