Doxorubicin-mediated apoptosis in glioma cells requires NFAT3
| Autor: | Jeffrey D. Klopfenstein, Dzung H. Dinh, Jasti S. Rao, Sreelatha Gopinath, Sravan K. Vanamala, Meena Gujrati |
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| Rok vydání: | 2009 |
| Předmět: |
Cell Survival
Poly ADP ribose polymerase Blotting Western Active Transport Cell Nucleus Fluorescent Antibody Technique Caspase 3 Apoptosis Biology Resting Phase Cell Cycle Article Cellular and Molecular Neuroscience Cell Movement Glioma Cell Line Tumor medicine polycyclic compounds Humans Caspase 10 Molecular Biology Pharmacology Cell Nucleus Antibiotics Antineoplastic NFATC Transcription Factors Reverse Transcriptase Polymerase Chain Reaction Tumor Necrosis Factor-alpha G1 Phase Cell migration NFAT Cell Biology medicine.disease Flow Cytometry Protein Transport Doxorubicin Receptors Tumor Necrosis Factor Type I Cancer research Molecular Medicine Tumor necrosis factor alpha RNA Interference Poly(ADP-ribose) Polymerases |
| Zdroj: | Cellular and molecular life sciences : CMLS. 66(24) |
| ISSN: | 1420-9071 |
| Popis: | Nuclear factor of activated T cells (NFAT), a family of transcription factors, has been implicated in many cellular processes, including some cancers. Here, we characterize, for the first time, the role of NFAT3 in doxorubicin (DOX)-mediated apoptosis, migration, and invasion in SNB19 and U87 glioma cells. This study demonstrates that the specific knockdown of NFAT3 results in a dramatic inhibition of the apoptotic effect induced by DOX and favors cell survival. Inhibition of NFAT3 activation by shNFAT3 (shNF3) significantly downregulated tumor necrosis factor (TNF)-alpha induction, its receptor TNFR1, caspase 10, caspase 3, and poly (ADP-ribose) polymerase, abrogating DOX-mediated apoptosis in glioma cells. DOX treatment resulted in NFAT3 translocation to the nucleus. Similarly, shNF3 treatment in SNB19 and U87 cells reversed DOX-induced inhibition of cell migration and invasion, as determined by wound healing and matrigel invasion assays. Taken together, these results indicate that NFAT3 is a prerequisite for the induction of DOX-mediated apoptosis in glioma cells. |
| Databáze: | OpenAIRE |
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