Mitochondrial Flash: Integrative Reactive Oxygen Species and pH Signals in Cell and Organelle Biology

Autor: Robert T. Dirksen, Wang Wang, Heping Cheng, Shey-Shing Sheu, Xianhua Wang, Lan Wei-LaPierre, Guohua Gong
Rok vydání: 2016
Předmět:
0301 basic medicine
genetic structures
Physiology
Clinical Biochemistry
Biosensing Techniques
Oxidative phosphorylation
Mitochondrion
Biochemistry
Antioxidants
Oxidative Phosphorylation
Cell Physiological Phenomena
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Superoxides
Organelle
Animals
Humans
Molecular Biology
Caenorhabditis elegans
General Environmental Science
biology
Superoxide
Cell Biology
Hydrogen-Ion Concentration
Forum Review Articles
biology.organism_classification
Mitochondria
Cell biology
Luminescent Proteins
030104 developmental biology
chemistry
mitochondrial fusion
General Earth and Planetary Sciences
Signal transduction
Reactive Oxygen Species
Oxidation-Reduction
Biomarkers
030217 neurology & neurosurgery
Function (biology)
Signal Transduction
Zdroj: Antioxidants & Redox Signaling
ISSN: 1557-7716
1523-0864
DOI: 10.1089/ars.2016.6739
Popis: Significance: Recent breakthroughs in mitochondrial research have advanced, reshaped, and revolutionized our view of the role of mitochondria in health and disease. These discoveries include the development of novel tools to probe mitochondrial biology, the molecular identification of mitochondrial functional proteins, and the emergence of new concepts and mechanisms in mitochondrial function regulation. The discovery of “mitochondrial flash” activity has provided unique insights not only into real-time visualization of individual mitochondrial redox and pH dynamics in live cells but has also advanced understanding of the excitability, autonomy, and integration of mitochondrial function in vivo. Recent Advances: The mitochondrial flash is a transient and stochastic event confined within an individual mitochondrion and is observed in a wide range of organisms from plants to Caenorhabditis elegans to mammals. As flash events involve multiple transient concurrent changes within the mitochondrion (e.g., superoxide, pH, and membrane potential), a number of different mitochondrial targeted fluorescent indicators can detect flash activity. Accumulating evidence indicates that flash events reflect integrated snapshots of an intermittent mitochondrial process arising from mitochondrial respiration chain activity associated with the transient opening of the mitochondrial permeability transition pore. Critical Issues: We review the history of flash discovery, summarize current understanding of flash biology, highlight controversies regarding the relative roles of superoxide and pH signals during a flash event, and bring forth the integration of both signals in flash genesis. Future Directions: Investigations using flash as a biomarker and establishing its role in cell signaling pathway will move the field forward. Antioxid. Redox Signal. 25, 534–549.
Databáze: OpenAIRE
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