Relationship between follicular dynamics and oocyte maturation during in vitro culture as a non-invasive sign of caprine oocyte meiotic competence
| Autor: | Matthew B. Wheeler, Felipe Zandonadi Brandão, C. Maside, A.C.A. Ferreira, Jesús Cadenas, V.M. Paes, A.P.R. Rodrigues, Benner Geraldo Alves, José Ricardo de Figueiredo, L.A. Vieira, Johanna Leiva-Revilla |
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| Rok vydání: | 2017 |
| Předmět: |
medicine.medical_specialty
In Vitro Oocyte Maturation Techniques Biology 03 medical and health sciences Follicle 0302 clinical medicine Food Animals Meiosis Ovarian Follicle Internal medicine Follicular phase medicine Animals Ovarian follicle Small Animals Cell Size 030219 obstetrics & reproductive medicine Estradiol Equine Goats 0402 animal and dairy science 04 agricultural and veterinary sciences Oocyte Antral follicle 040201 dairy & animal science Chromatin medicine.anatomical_structure Endocrinology Oocytes Animal Science and Zoology Female |
| Zdroj: | Theriogenology. 107 |
| ISSN: | 1879-3231 |
| Popis: | The search for non-invasive signs of oocyte meiotic competence is very important for the development of in vitro follicle culture (IVFC) systems. The aims of the present study were: (1) to investigate the effect of in vitro maturation (IVM) of in vivo grown goat COCs, in group or individually, on oocyte chromatin configuration (Experiment 1), and (2) the influence of IVFC period (12 vs. 18 days) on the ability of the oocyte to resume meiosis immediately after IVFC (before in vitro maturation; IVM), or after IVM (Experiment 2). In experiment 1, in vivo grown cumulus-oocyte complexes (COCs) were submitted to IVM in groups (10 COCs/100 μL-drop) or individually (1 COC/10 μL-drop), and chromatin configuration was assessed. In experiment 2, isolated follicles were individually cultured for 12 or 18 days, and submitted to individual IVM afterwards. The following end points were evaluated: follicular growth and morphology, oocyte diameter, viability and chromatin configuration, as well as individual follicular estradiol production. Similar maturation rates were obtained between in vivo grown COCs matured individually and in groups (66.7% vs. 63.6%, respectively) (Experiment 1). Only after 18 days of IVFC, oocytes were able to grow during IVM, reaching a mean oocyte diameter of 119 μm. Also, this treatment produced the highest rate of metaphase II oocytes (46.2% out of the total number of cultured follicles). Finally, it was observed that follicles with a daily growth rate7.1 μm/day (fast-growing) and that reached at least 600 μm in diameter, were more likely (P 0.05) to produce oocytes capable of attaining MII. In conclusion, caprine oocytes can be individually matured in vitro, as efficiently as in groups. This result was essential to pair in vitro follicle development and in vitro oocyte maturation with specific individual follicles. Using this approach, it was possible to establish non-invasive signs for the efficiency of IVFC based on follicle daily growth rate and diameter, and oocyte diameter: follicle daily growth7 μm, follicle diameter of at least 600 μm, and oocyte diameter ≥120 μm. In addition, 18 days seems to be the most suitable culture time for caprine early antral follicles. |
| Databáze: | OpenAIRE |
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