Fluticasone reduces IL-6 and IL-8 production of cystic fibrosis bronchial epithelial cellsviaIKK-β kinase pathway
| Autor: | Edith Puchelle, Jacky Jacquot, Caroline Majer-Teboul, Daniel Dusser, Sandie Escotte, Olivier Tabary |
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| Přispěvatelé: | Dynamique cellulaire et moléculaire de la muqueuse respiratoire, Université de Reims Champagne-Ardenne (URCA)-IFR53-Institut National de la Santé et de la Recherche Médicale (INSERM), Service de pneumologie [CHU Cochin], Hôpital Cochin [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Department of clinical pharmacology (GSK), Laboratoire GlaxoSmithKline, Birembaut, Philippe, Université de Reims Champagne-Ardenne (URCA) - IFR53 - Institut National de la Santé et de la Recherche Médicale (INSERM), Assistance publique - Hôpitaux de Paris (AP-HP) - CHU Cochin [AP-HP] |
| Rok vydání: | 2003 |
| Předmět: |
Cystic Fibrosis
MESH: I-kappa B Proteins medicine.medical_treatment [SDV.MHEP.PSR]Life Sciences [q-bio]/Human health and pathology/Pulmonology and respiratory tract Cystic fibrosis Medicine Chemokine CCL5 Cells Cultured biology MESH: Bronchi MESH: Enzyme-Linked Immunosorbent Assay Interleukin MESH: Chemokines MESH: Case-Control Studies Bronchodilator Agents I-kappa B Kinase medicine.anatomical_structure Cytokine MESH: Epithelial Cells I-kappa B Proteins Chemokines Signal transduction MESH: Cells Cultured Pulmonary and Respiratory Medicine medicine.medical_specialty MESH: Cystic Fibrosis Blotting Western Bronchi Enzyme-Linked Immunosorbent Assay Respiratory Mucosa Protein Serine-Threonine Kinases MESH: Analysis of Variance Internal medicine Humans MESH: Blotting Western MESH: I-kappa B Kinase Interleukin 8 Interleukin 6 Analysis of Variance MESH: Humans Lung Interleukin-6 Tumor Necrosis Factor-alpha business.industry Interleukin-8 I-Kappa-B Kinase Epithelial Cells medicine.disease Androstadienes Endocrinology Case-Control Studies MESH: Androstadienes biology.protein Cancer research Fluticasone [SDV.MHEP.PSR] Life Sciences [q-bio]/Human health and pathology/Pulmonology and respiratory tract MESH: Bronchodilator Agents business |
| Zdroj: | European Respiratory Journal European Respiratory Journal, European Respiratory Society, 2003, 21 (4), pp.574-81 |
| ISSN: | 1399-3003 0903-1936 |
| DOI: | 10.1183/09031936.03.00031803 |
| Popis: | FREE FULL TEXT http://erj.ersjournals.com/cgi/content/full/21/4/574; Inhaled fluticasone propionate (FP) is widely used to reduce pulmonary inflammation in chronic obstructive pulmonary disease, but the potential effects of FP on airway epithelial cells from patients with cystic fibrosis (CF) are unknown. In CF disease, a nonregulated inflammatory lung response occurs through exaggerated nuclear factor (NF)-kappaB activation and elevated pro-inflammatory cytokines production by airway epithelial cells. To determine whether FP reduces cytokine production in bronchial epithelial cells via NF-kappaB, the authors investigated the nonstimulated and the Pseudomonas aeruginosa lipopolysaccharide (LPS) stimulated production of NF-kappaB-dependent interleukin (IL)-6, IL-8 and RANTES (regulated on activation, T-cell expressed and secreted) along with the activation of NF-kappaB in non-CF and CF human bronchial gland epithelial cells. It was demonstrated that a relevant concentration of FP (10(-8) M) inhibited constitutive and P. aeruginosa LPS-induced IL-6 and IL-8 production of non-CF and CF bronchial epithelial cells. Interestingly, the expression of two IkappaB kinases (IKK)-alpha/beta, the degradation of cytosolic IkappaB-beta inhibitor and the NF-kappaB deoxyribonucleic acid binding activity were markedly reduced after FP treatment in both CF and non-CF bronchial epithelial cells. It was shown by the authors that fluticasone propionate exerts an anti-inflammatory effect by blocking a signal transduction leading to a reduced level of IkappaB-alpha/beta kinases in bronchial epithelial cells. In particular the strong effect on the IkappaB-beta kinase, which is known to be elevated in bronchial epithelial cells in cystic fibrosis patients, was observed. |
| Databáze: | OpenAIRE |
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