The Wnt/planar cell polarity signaling pathway contributes to the integrity of tight junctions in brain endothelial cells
| Autor: | Cédric Artus, Kayathiri Ganeshamoorthy, Nicole Ziegler, Fabienne Glacial, Stefan Liebner, Pierre-Olivier Couraud, Thomas Guilbert, Maeva Godet |
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| Rok vydání: | 2013 |
| Předmět: |
Cell Culture Techniques
Cell Cycle Proteins Biology Blood–brain barrier Real-Time Polymerase Chain Reaction Permeability Wnt-5a Protein Cell Line Tight Junctions Proto-Oncogene Proteins Cell polarity medicine Humans RNA Small Interfering Wnt Signaling Pathway Protein Kinase C Adaptor Proteins Signal Transducing Tight junction Wnt signaling pathway LRP6 Cell Polarity Endothelial Cells Membrane Proteins LRP5 Cell biology Endothelial stem cell Wnt Proteins medicine.anatomical_structure Neurology Microscopy Fluorescence Blood-Brain Barrier Original Article Neurology (clinical) Signal transduction Cardiology and Cardiovascular Medicine |
| Zdroj: | Journal of Cerebral Blood Flow & Metabolism |
| ISSN: | 1559-7016 |
| Popis: | Wnt morphogens released by neural precursor cells were recently reported to control blood–brain barrier (BBB) formation during development. Indeed, in mouse brain endothelial cells, activation of the Wnt/ β-catenin signaling pathway, also known as the canonical Wnt pathway, was shown to stabilize endothelial tight junctions (TJs) through transcriptional regulation of the expression of TJ proteins. Because Wnt proteins activate several distinct β-catenin-dependent and independent signaling pathways, this study was designed to assess whether the noncanonical Wnt/Par/aPKC planar cell polarity (PCP) pathway might also control TJ integrity in brain endothelial cells. First we established, in the hCMEC/D3 human brain endothelial cell line, that the Par/aPKC PCP complex colocalizes with TJs and controls apicobasal polarization. Second, using an siRNA approach, we showed that the Par/aPKC PCP complex regulates TJ stability and reassembling after osmotic shock. Finally, we provided evidence that Wnt5a signals in hCMEC/D3 cells through activation of the Par/aPKC PCP complex, independently of the Wnt canonical β-catenin-dependent pathway and significantly contributes to TJ integrity and endothelial apicobasal polarity. In conclusion, this study suggests that the Wnt/Par/aPKC PCP pathway, in addition to the Wnt/ β-catenin canonical pathway, is a key regulator of the BBB. |
| Databáze: | OpenAIRE |
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