JMY expression by Sertoli cells contributes to mediating spermatogenesis in mice
| Autor: | Zhide Ding, Ran Zhao, Yan Yan, Xuening Dang, Yue Liu, Yimei Xu, Jiaying Fan |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Male
0301 basic medicine endocrine system Proteome Endocytic cycle Actin filament organization Cell Cycle Proteins Biology Biochemistry Mice 03 medical and health sciences 0302 clinical medicine medicine Animals Spermatogenesis Cytoskeleton Molecular Biology Infertility Male Sperm motility Mice Knockout Sertoli Cells urogenital system Cell Biology Sertoli cell Actin cytoskeleton Spermatozoa Cell biology Mice Inbred C57BL Vesicular transport protein Actin Cytoskeleton 030104 developmental biology medicine.anatomical_structure Endocytic vesicle 030220 oncology & carcinogenesis Trans-Activators Female |
| Zdroj: | The FEBS Journal. 287:5478-5497 |
| ISSN: | 1742-4658 1742-464X |
| Popis: | Sertoli cells are crucial for spermatogenesis in the seminiferous epithelium because their actin cytoskeleton supports vesicular transport, cell junction formation, protein anchoring, and spermiation. Here, we show that a junction-mediating and actin-regulatory protein (JMY) affects the blood-tissue barrier (BTB) function through remodeling of the Sertoli cell junctional integrity and it also contributes to controlling endocytic vesicle trafficking. These functions are critical for the maintenance of sperm fertility since loss of Sertoli cell-specific Jmy function induced male subfertility in mice. Specifically, these mice have (a) impaired BTB integrity and spermatid adhesion in the seminiferous tubules; (b) high incidence of sperm structural deformity; and (c) reduced sperm count and poor sperm motility. Moreover, the cytoskeletal integrity was compromised along with endocytic vesicular trafficking. These effects impaired junctional protein recycling and reduced Sertoli cell BTB junctional integrity. In addition, JMY interaction with actin-binding protein candidates α-actinin1 and sorbin and SH3 domain containing protein 2 was related to JMY activity, and in turn, actin cytoskeletal organization. In summary, JMY affects the control of spermatogenesis through the regulation of actin filament organization and endocytic vesicle trafficking in Sertoli cells. |
| Databáze: | OpenAIRE |
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