The Dissociation of Human Serum 7S and Bovine Colostrum Immune Globulins into Subunits by Reduction-Alkylation-Succinylation

Autor:	A, Bezkorovainy, R, Zschocke, D, Grohlich
Rok vydání:	1970
Předmět:	Immunodiffusion Colostrum Immune Sera Immunochemistry Immunology Transferrin Succinates Anhydrides Immunoglobulin G Animals Humans Immunology and Allergy Cattle Serum Globulins Amino Acids Antigens Immunoelectrophoresis Ultracentrifugation Mercaptoethanol
Zdroj:	The Journal of Immunology. 104:648-655
ISSN:	1550-6606 0022-1767
Popis:	Human 7S and bovine colostral immunoglobulins were succinylated and reduced-alkylated, and their subunits were separated by chromatography on Sephadex G-200 columns. Human 7S immunoglobulin yielded heavy chains with a molecular weight of 55,700 and light chains with a molecular weight of 22,300. Bovine colostral immunoglobulins gave heavy and light chains with molecular weights of 49,500 and 25,200, respectively. Since the molecular weight of the colostral immunoglobulin of 166,000 is not accounted for by two heavy and two light chains, the possibility is raised that another subunit possibly exists in colostral immunoglobulin. The succinylated proteins lost their ability to form precipitin lines with antisera to native bovine and human immunoglobulins, but they formed precipitin lines in the double diffusion system with an antiserum to the succinyl hapten residues.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::312f4a53cc2acc287bc8f83a89a205fd https://doi.org/10.4049/jimmunol.104.3.648 Zobrazit plný text záznamu