Mechanism and optimization of supramolecular complexation‐enhanced fluorescence spectroscopy for the determination of SN‐38 in plasma and cells
Autor: | Kena Zhao, Xian Sun, Senlin Shi, Ting Xiong, Rui Yang, Huimin Sun, Tao Guo, Li Wu, Xiaohong Ren, Jiwen Zhang |
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Rok vydání: | 2020 |
Předmět: |
Kinetics
Biophysics Supramolecular chemistry 02 engineering and technology Irinotecan 01 natural sciences Fluorescence spectroscopy chemistry.chemical_compound Animals Tissue Distribution Carboxylate chemistry.chemical_classification Detection limit Cyclodextrins Cyclodextrin 010401 analytical chemistry 021001 nanoscience & nanotechnology Fluorescence Rats 0104 chemical sciences Spectrometry Fluorescence Pharmaceutical Preparations chemistry Chemistry (miscellaneous) Physical chemistry 0210 nano-technology Quantitative analysis (chemistry) |
Zdroj: | Luminescence. 36:531-542 |
ISSN: | 1522-7243 1522-7235 |
DOI: | 10.1002/bio.3973 |
Popis: | Quantitative detection of two different forms of SN-38 in biological samples is, currently, cumbersome and difficult. A revisit to the mechanism of supramolecular complexation-enhanced fluorescence spectroscopy helps to optimize the determination of SN-38 in plasma and the cellular pharmacokinetics in A549 cells based on the supramolecular complexation. Firstly, the inclusion mechanism dominated by thermodynamic constants was determined by measuring kinetic/thermodynamic parameters (kon , koff , ΔG, ΔH, ΔS). On this basis, the best effect of fluorescence sensitization was optimized through screening the interaction conditions (cyclodextrin species and concentrations, drug levels, temperature, pH of the buffer, and reaction time). Furthermore, the proportional relationship between the concentration of the inclusion complex and the fluorescence intensity was confirmed. Finally, a highly sensitive, selective spectrofluorimetric method was established and validated for quantitative analysis of the lactone and carboxylate molecular states of SN-38 plasma levels in rats and cell membrane transfer kinetics in A549 cell lines. The limits of detection for the lactone and carboxylate forms in plasma were found to be 0.44 ng·ml-1 and 0.28 ng·ml-1 , respectively. Precision and accuracy met the requirements of biological samples analysis. The proposed detection method provided a reference for elucidating the biodistribution of SN-38. |
Databáze: | OpenAIRE |
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