Comparative Evaluation of Profiles of Antibodies to Mycobacterial Capsular Polysaccharides in Tuberculosis Patients and Controls Stratified by HIV Status
| Autor: | Elisabeth R. Jenny-Avital, Arturo Casadevall, Katherine Sosa, Xian Yu, Jacqueline M. Achkar, Rafael Prados-Rosales |
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| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
Microbiology (medical)
Adult Lipopolysaccharides Male Tuberculosis Clinical Biochemistry Immunology HIV Infections Immunoglobulin G Serology Mycobacterium tuberculosis Mannans Antigen Bacterial Proteins Diagnostic Laboratory Immunology medicine Immunology and Allergy Humans Serologic Tests Glucans Bacterial Capsules Antigens Bacterial Lipoarabinomannan biology Malate Synthase virus diseases Middle Aged biology.organism_classification medicine.disease Virology Isotype Antibodies Bacterial biology.protein Female Antibody Biomarkers |
| Popis: | Despite the complexity of tuberculosis (TB) serology, antibodies (Abs) remain attractive biomarkers for TB. Recent evidence of a mycobacterial capsule that consists mainly of the polysaccharides arabinomannan (AM) and glucan provides new options for serologic targets. For this study, Ab responses to AM and glucan for 47 U.S. TB patients (33 HIV negative [HIV − ], 14 HIV positive [HIV + ]), 42 healthy controls, and 38 asymptomatic HIV + controls were evaluated by enzyme-linked immunosorbent assays (ELISAs). The results were compared with Ab responses to the mycobacterial glycolipid cell wall antigen lipoarabinomannan (LAM) and to the proteins malate synthase (MS) and MPT51. We found that the main immunoglobulin (Ig) isotype response to polysaccharides was IgG, predominantly of subclass IgG2. IgG responses to AM were significantly higher for HIV − and HIV + TB cases than for controls ( P , − than for HIV + TB cases ( P , − and HIV + cases ( P , 0.01 and 0.02, respectively). In both TB groups, titers of Ab to glucan were significantly lower than titers of Ab to AM ( P , − TB patients, while they correlated significantly in HIV + TB patients ( P , 0.01 and 0.05, respectively). We conclude that Ab responses to AM could contribute to the serodiagnosis of TB, especially for HIV − TB patients. This study also provides new and important insights into the differences in the profiles of Abs to mycobacterial antigens between HIV − and HIV + TB patients. |
| Databáze: | OpenAIRE |
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