Cerebral endothelial cell culture II. Adenylate cyclase response to prostaglandins and their interaction with the adrenergic system
| Autor: | Maria Spatz, Joliet Bembry, Irina L. Karnushina |
|---|---|
| Rok vydání: | 1983 |
| Předmět: |
medicine.medical_specialty
Endothelium Alpha (ethology) Adrenergic Adenylate kinase Biology Cyclase General Biochemistry Genetics and Molecular Biology Norepinephrine (medication) Norepinephrine chemistry.chemical_compound Phentolamine Internal medicine medicine Animals General Pharmacology Toxicology and Pharmaceutics Cells Cultured Forskolin Prostaglandins E Brain General Medicine Epoprostenol Rats Receptors Adrenergic medicine.anatomical_structure Endocrinology chemistry Prostaglandins lipids (amino acids peptides and proteins) Adenylyl Cyclases medicine.drug |
| Zdroj: | Life Sciences. 32:1427-1435 |
| ISSN: | 0024-3205 |
| DOI: | 10.1016/0024-3205(83)90907-4 |
| Popis: | The response of endothelial adenylate cyclase (AC) to prostaglandins (PGE 1 , PGE 2 , PGF 1α , PGF 2α , PGD 2 and PGI 2 ) and the relationship of PGE 2 to adrenergic systems were investigated in cerebrovascular endothelial cultures. E-type prostaglandins and PGI 2 were more effective in stimulating endothelial AC (EC 50 = 3 × 10 −7 M, and 3 × 10 −7 M, respectively) than prostaglandins of the F-series and PGD 2 which activated AC at high doses only. A modulation of endothelial AC response to either PGE 2 or norepinephrine (NE) was observed in the presence of both agents in the system. It was manifested by a dose-dependent NE inhibition of the PGE 2 -stimulated formation of cAMP, which was partially restored by phentolamine. Alpha and β-adrenergic agonists (α, clonidine and 6-fluoronorepinephrine; β, isoproterenol) also partly blocked while forskolin and PGE 2 synergistically stimulated the production of cAMP in the endothelial cultures. These findings strongly suggest that the interaction of prostaglandins and α- and β-adrenergic agonists with the AC system in cerebrovascular endothelium may play a role in the regulation of the cerebral microcirculation and/or blood pressure. |
| Databáze: | OpenAIRE |
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