Purification, structure characterization and antioxidant activity of polysaccharides from Saposhnikovia divaricata
Autor: | Jie Hao, Yao Meng, Jie Xue, Nai-Yu Xu, Du-Xin Li, Zhenqing Zhang, Lin Yi, Lei Chen |
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Rok vydání: | 2019 |
Předmět: |
Antioxidant
food.ingredient Cell Survival medicine.medical_treatment Saposhnikovia divaricata Polysaccharide Plant Roots 01 natural sciences Antioxidants Mice 03 medical and health sciences 0302 clinical medicine food Polysaccharides Arabinogalactan Drug Discovery medicine Animals Monosaccharide Food science chemistry.chemical_classification 010405 organic chemistry Chemistry Macrophages General Medicine 0104 chemical sciences RAW 264.7 Cells Complementary and alternative medicine 030220 oncology & carcinogenesis Herb Apiaceae Drugs Chinese Herbal |
Zdroj: | Chinese Journal of Natural Medicines. 17:792-800 |
ISSN: | 1875-5364 |
DOI: | 10.1016/s1875-5364(19)30096-2 |
Popis: | Polysaccharide from traditional Chinese herb, Saposhnikovia divaricata (Turcz.) Schischk. (SD) was extracted, fractionated and characterized in this work. Four fractions were prepared. Their molecular weight, monosaccharide compositions, linkage modes and structural properties were characterized with SEC-MALS-RI, HPAEC-PAD, GC-MS and NMR. SDP1 was assigned as a 1, 4-α-glucan with small amount of O-6 linked branches. SDP2 contained a big amount of the 1, 4-α-glucan and a small amount of arabinogalactan, while SDP3 possessed relatively lower amount of the 1, 4-α-glucan and a big amount of the arabinogalactan. SDP4 was defined as a pectic arabinogalactan. Four fractions showed antioxidant activities in both molecular and cellular levels and their activity was ranked as SDP4 ≈ SDP3>SDP2>SDP1. The 1, 4-α-glucan in SDP1 had the weakest, while SDP3 and SDP4 showed similar and the highest antioxidant activity. The arabinogalactan was the major component of both SDP3 and SDP4, which significantly contributed to the antioxidant activity of SDP. |
Databáze: | OpenAIRE |
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