A quantitative and interspecific test for biological activity of anti-Müllerian hormone: the fetal ovary aromatase assay
| Autor: | Richard L. Cate, R B Pepinsky, Claude Pieau, S Ghaffari, B. Vigier, Nathalie Josso, N di Clemente |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Anti-Mullerian Hormone
Male endocrine system medicine.medical_specialty Gonad endocrine system diseases Ovary Aromatase Organ Culture Techniques Species Specificity Internal medicine Testis Cyclic AMP medicine Animals Humans Mullerian Ducts Molecular Biology Glycoproteins Fetus Sexual differentiation biology urogenital system Rats Inbred Strains Embryo Anti-Müllerian hormone Growth Inhibitors female genital diseases and pregnancy complications Rats Testicular Hormones medicine.anatomical_structure Endocrinology Depression Chemical biology.protein Biological Assay Cattle Electrophoresis Polyacrylamide Gel Female Developmental Biology Hormone |
| Zdroj: | Development. 114:721-727 |
| ISSN: | 1477-9129 0950-1991 |
| DOI: | 10.1242/dev.114.3.721 |
| Popis: | Anti-Müllerian hormone (AMH), also known as Müllerian-inhibiting substance or factor, has previously been shown to sex-reverse the steroidogenic pattern of fetal mammalian ovaries through repression of aromatase biosynthesis. Study of the ontogeny of the response of cyclic AMP-stimulated aromatase activity of rat fetal ovaries to AMH has allowed us to develop a quantitative biossay for the hormone. Linear responses as a function of the logarithm of AMH concentration were observed over ranges of 0.2 -7.5 μg/ml for the bovine protein and 0.15 -2 μg/ml for the human protein, with a maximal decrease in aromatase activity of 90% for both proteins. Under the same in vitro conditions, AMH treatment did not affect cyclic AMP-stimulated fetal rat testicular aromatase activity. Partially purified chick AMH also decreased rat ovarian aromatase activity, allowing us to use this test to study AMH ontogeny in chick gonads. Analysis of the species specificity of AMH repression of ovarian aromatase activity indicated that turtle and rat fetal ovaries responded to AMH of other vertebrate classes, whereas aromatase activity of chick embryo ovaries could be repressed only by the homospecific hormone. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|