Characterization of Pepsinogen C as a Potential Biomarker for Gastric Cancer Using a Histo-Proteomic Approach
| Autor: | von Eggeling F, Richter Kk, Bettina Schimmel, Christian Melle, Daffner W, Annett Bleul, Günther Ernst, Uwe Claussen, Merten Hommann, Utz Settmacher, Roland Kaufmann |
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| Rok vydání: | 2005 |
| Předmět: |
Proteomics
Proteome Pepsinogen C Protein Array Analysis Biology Bioinformatics Peptide Mapping Biochemistry Epithelium Mass Spectrometry Stomach Neoplasms Cell Line Tumor Biomarkers Tumor medicine Humans Electrophoresis Gel Two-Dimensional Microdissection Lasers Cancer General Chemistry medicine.disease Immunohistochemistry Molecular biology Gene Expression Regulation Neoplastic Gastric Mucosa Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Potential biomarkers Gastric epithelium Biomarker (medicine) Biomarkers |
| Zdroj: | Journal of Proteome Research. 4:1799-1804 |
| ISSN: | 1535-3907 1535-3893 |
| DOI: | 10.1021/pr050123o |
| Popis: | We analyzed 74 cryostat sections of central gastric tumor, tumor margin, and normal gastric epithelium using ProteinChip Arrays and SELDI-TOF MS. One peak was significantly down-regulated in tumor tissue (P = 1.43 × 10-6) and identified as pepsinogen C using MS/MS analysis and immunodepletion. This signal was further characterized by immunohistochemistry. This work demonstrates that differentially expressed signals can be identified and assessed using a proteomic approach comprising tissue-microdissection, protein profiling, and immunohistochemistry. Keywords: gastric cancer • proteomics • biomarker • ProteinChip technology • SELDI • microdissection • immunohistochemistry |
| Databáze: | OpenAIRE |
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