Identification and DNA Marker Development for a Wheat-Leymus mollis 2Ns (2D) Disomic Chromosome Substitution

Autor:	Xianbo Feng, Xin Du, Siwen Wang, Pingchuan Deng, Yongfu Wang, Lihui Shang, Zengrong Tian, Changyou Wang, Chunhuan Chen, Jixin Zhao, Wanquan Ji
Jazyk:	angličtina
Rok vydání:	2022
Předmět:	Inorganic Chemistry Organic Chemistry food and beverages General Medicine Leymus mollis molecular cytogenetics SLAF-seq stripe rust wheat 55K array Physical and Theoretical Chemistry Molecular Biology Spectroscopy Catalysis Computer Science Applications
Zdroj:	International Journal of Molecular Sciences; Volume 23; Issue 5; Pages: 2676
ISSN:	1422-0067
DOI:	10.3390/ijms23052676
Popis:	Leymus mollis (2n = 4x = 28, NsNsXmXm), a wild relative of common wheat (Triticum aestivum L.), carries numerous loci which could potentially be used in wheat improvement. In this study, line 17DM48 was isolated from the progeny of a wheat and L. mollis hybrid. This line has 42 chromosomes forming 21 bivalents at meiotic metaphase I. Genomic in situ hybridization (GISH) demonstrated the presence of a pair chromosomes from the Ns genome of L. mollis. This pair substituted for wheat chromosome 2D, as shown by fluorescence in situ hybridization (FISH), DNA marker analysis, and hybridization to wheat 55K SNP array. Therefore, 17DM48 is a wheat-L. mollis 2Ns (2D) disomic substitution line. It shows longer spike and a high level of stripe rust resistance. Using specific-locus amplified fragment sequencing (SLAF-seq), 13 DNA markers were developed to identify and trace chromosome 2Ns of L. mollis in wheat background. This line provides a potential bridge germplasm for genetic improvement of wheat stripe rust resistance.
Databáze:	OpenAIRE
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