Versatile Near-Infrared Super-Resolution Imaging of Amyloid Fibrils with the Fluorogenic Probe CRANAD-2

Autor:	Joaquim Torra, Felipe Viela, Diego Megías, Begoña Sot, Cristina Flors
Jazyk:	angličtina
Rok vydání:	2022
Předmět:	Amyloid Curcumin Organic Chemistry General Chemistry Microscopy Atomic Force Single Molecule Imaging Catalysis Fluorescent Dyes
Zdroj:	Repositorio Institucional del Instituto Madrileño de Estudios Avanzados en Nanociencia instname
Popis:	CRANAD-2 is a fluorogenic curcumin derivative used for near-infrared detection and imaging in vivo of amyloid aggregates, which are involved in neurodegenerative diseases. We explore the performance of CRANAD-2 in two super-resolution imaging techniques, namely stimulated emission depletion (STED) and single-molecule localization microscopy (SMLM), with markedly different fluorophore requirements. By conveniently adapting the concentration of CRANAD-2, which transiently binds to amyloid fibrils, we show that it performs well in both techniques, achieving a resolution in the range of 45-55 nm. Correlation of SMLM with atomic force microscopy (AFM) validates the resolution of fine features in the reconstructed super-resolved image. The good performance and versatility of CRANAD-2 provides a powerful tool for near-infrared nanoscopic imaging of amyloids in vitro and in vivo.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3116da8aefdf7a49e2695b78ac15d9f0 http://hdl.handle.net/20.500.12614/2811 Zobrazit plný text záznamu