Genetics of Adaptation of the Ascomycetous Fungus Podospora anserina to Submerged Cultivation
| Autor: | Ksenia R. Safina, Viktoria N Moskalenko, Georgii A. Bazykin, Maria D. Logacheva, Olga A. Vakhrusheva, Alexey S. Kondrashov, Elena S Glagoleva, Tatiana V. Neretina, Olga A Kudryavtseva, Aleksey A. Penin |
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| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
Population Mycology adaptation Fungus Biology 010603 evolutionary biology 01 natural sciences Podospora anserina Evolution Molecular Fungal Proteins 03 medical and health sciences INDEL Mutation Podospora positive selection Genetics experimental evolution Allele education Gene Alleles submerged cultivation Ecology Evolution Behavior and Systematics 030304 developmental biology 0303 health sciences education.field_of_study Experimental evolution Genetic Variation biology.organism_classification Phenotype Genome Fungal Adaptation Research Article |
| Zdroj: | Genome Biology and Evolution |
| ISSN: | 1759-6653 |
| Popis: | Podospora anserina is a model ascomycetous fungus which shows pronounced phenotypic senescence when grown on solid medium but possesses unlimited lifespan under submerged cultivation. In order to study the genetic aspects of adaptation of P. anserina to submerged cultivation, we initiated a long-term evolution experiment. In the course of the first 4 years of the experiment, 125 single-nucleotide substitutions and 23 short indels were fixed in eight independently evolving populations. Six proteins that affect fungal growth and development evolved in more than one population; in particular, in the G-protein alpha subunit FadA, new alleles fixed in seven out of eight experimental populations, and these fixations affected just four amino acid sites, which is an unprecedented level of parallelism in experimental evolution. Parallel evolution at the level of genes and pathways, an excess of nonsense and missense substitutions, and an elevated conservation of proteins and their sites where the changes occurred suggest that many of the observed fixations were adaptive and driven by positive selection. |
| Databáze: | OpenAIRE |
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