Hypericum Humifusum Leaves Attenuates Hepatic Ischemia-Reperfusion Injury in a Rat Model

Autor: Nadia Kourda, Sarra Bacha, Emna Gaïes, Issam Salouage, Nadia Jebabli, Anis Klouz, Sameh Trabelsi, Henda Ferchichi
Jazyk: angličtina
Rok vydání: 2018
Předmět:
Male
Antioxidant
Necrosis
medicine.medical_treatment
Specialties of internal medicine
Apoptosis
Pharmacology
Plant aqueous extract
Antioxidants
chemistry.chemical_compound
0302 clinical medicine
Malondialdehyde
Gallic acid
Hypericum humifusum
biology
Liver Diseases
Alanine Transaminase
General Medicine
Liver
RC581-951
Reperfusion Injury
030220 oncology & carcinogenesis
030211 gastroenterology & hepatology
medicine.symptom
Hypericum
03 medical and health sciences
Antioxidant activity
medicine
Animals
Aspartate Aminotransferases
Rats
Wistar
Total phenols
Plants
Medicinal
Hepatology
Plant Extracts
business.industry
Vitamin E
Polyphenols
biology.organism_classification
medicine.disease
Plant Leaves
Disease Models
Animal
Oxidative Stress
chemistry
Cytoprotection
Lipid Peroxidation
business
Reperfusion injury
Biomarkers
Phytotherapy
Zdroj: Annals of Hepatology, Vol 17, Iss 1, Pp 144-152 (2018)
ISSN: 1665-2681
Popis: Introduction. Effective prevention strategies require specific actions during the different phases of ischemia-reperfusion (I-R) injury. The objective of our study is to evaluate the effect of aqueous extract of Hypericum humifusum leaves (HHL) on liver I-R model in Rat. Material and methods. Animals were subjected to 90 min of hepatic ischemia followed by reperfusion (120 min). HHL extract (25 mg/mL/kg) was injected 15 min before reperfusion. To evaluate the effect of HHL extract on I-R, we have monitored transaminases levels, Malondialdehyde (MDA) concentration, histological lesions (apoptosis and necrosis) and compared the results to a reference oxidant vitamin E. Results. The determination of total phenol extracts of HHL was 59.91 ± 0.35 mg of Gallic Acid/g dry plant material with higher antioxidant activity (91.73% ± 1.67) compared to vitamin E (87.42%). Using aqueous extract of HHL, we noted a significant decrease of AST and ALT [1129 UI (585/1995) and 768 UI (335/1375)] compared to no-treated group [5,585.5 UI (5,035/12,070) and 8,099.5 UI (5,040/12,326)] as a decrease in MDA content [85.7% protection (50.9/91.5)]. HHL extract reduce the damage induced by I-R of 48.7% (27/48.7) and 96.1% (95.7/96.5) for necrosis and apoptosis lesions respectively. Conclusion. HHL aqueous extract have potential to protect liver from the damage effect induced by I-R better than vitamin E solution.
Databáze: OpenAIRE
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