Evaluation of liver fibrosis by investigation of hepatic parenchymal perfusion using contrast-enhanced ultrasound: An animal study
| Autor: | George L. Tipoe, Yongping Zheng, Yanping Huang, Michael Ying, Gina Leung, Thomas Y.H. Lau, Esther S.T. Lee, Queeny W.H. Yuen |
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| Rok vydání: | 2012 |
| Předmět: |
Liver Cirrhosis
Male Pathology medicine.medical_specialty medicine.medical_treatment Intraperitoneal injection Contrast Media Sensitivity and Specificity Severity of Illness Index Rats Sprague-Dawley Random Allocation chemistry.chemical_compound Reference Values Fibrosis Parenchyma medicine Animals Radiology Nuclear Medicine and imaging Ultrasonography Doppler Color Stage (cooking) Carbon Tetrachloride business.industry Biopsy Needle Ultrasound Image Enhancement medicine.disease Immunohistochemistry Rats Perfusion Disease Models Animal chemistry Carbon tetrachloride business Contrast-enhanced ultrasound |
| Zdroj: | Journal of Clinical Ultrasound. 40:462-470 |
| ISSN: | 0091-2751 |
| DOI: | 10.1002/jcu.21969 |
| Popis: | Purpose: To investigate the value of assessing the hepatic parenchymal perfusion in contrast-enhanced ultrasound (CEUS) for evaluating liver fibrosis, using an animal model. Methods: Seventy Sprague–Dawley rats were divided into experimental (n = 35) and control (n = 35) groups. In the experimental group, liver fibrosis was induced by intraperitoneal injection of carbon tetrachloride. CEUS of the liver was performed at a 2-week interval for 14 weeks. Signal intensity of liver parenchyma was analyzed with time-intensity curves. Histologic examination of liver specimens of the animals was performed to assess the fibrosis stage. Results: The peak signal intensity of hepatic parenchymal perfusion in stage 2–3 fibrosis was significantly lower than that in stage 0–1. The time to peak intensity of hepatic parenchymal perfusion was significantly longer in the experimental group than the control group, and in the stage 3 fibrosis than in stages 0–2 fibrosis. Using time to peak intensity of hepatic parenchymal perfusion to distinguish stage 3 fibrosis and stages 0–2 fibrosis, the optimum cutoff was 75,000 milliseconds with the sensitivity and specificity of 67% and 78%, respectively. Conclusions: This animal study showed that CEUS has the potential to be a complementary imaging tool in the evaluation of liver fibrosis. © 2012 Wiley Periodicals, Inc. J Clin Ultrasound 40:462–470, 2012 |
| Databáze: | OpenAIRE |
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