| Popis: |
Lipase from Pseudomonas glumae has been purified and crystallized in two forms, using the hanging drop method of vapour diffusion at 4 °C and 15 °C. Both forms grew at pH 9.0 from 0.1 m -Tris buffer in the presence of 10% (v/v) acetone. Form 1 was crystallized from 27 to 29% polyethylene glycol in the presence of less than 0.5% (v/v) n-dodecyl-β- d -glucopyranoside Form 2 was grown from 17 to 19% ammonium sulphate in the presence of 1% n-octyl-β- d -glucopyranoside. Form 1 is orthorhombic with space group P212121, and cell dimensions of a = 158.1 A , b = 158.6 A , c = 63.4 A , Form 2 is tetragonal with space group P41212 (or P43212) and cell dimensions of a = 89.3 A , c = 180.4 A . Form 1 probably has four molecules per asymmetric unit and diffracts to at least 2.5 A. Form 2 has two molecules per asymmetric unit and diffracts to at least 3.0 A. |
